We present a theoretical analysis of the image formation in structured illumination wide-field fluorescence microscopy (SIWFFM). We show that the optically sectioned images obtained with this approach possess the optical sectioning strengths comparable to those obtained with the confocal microscope. We further show that the transfer function behaviour is directly comparable to that of the true confocal instrument. The theoretical considerations are compared with and confirmed by experimental results.

Dejan Karadaglić

Tony Wilson

Micron (Oxford, England : 1993)

MICRON 是一个跨学科论坛，适用于所有涉及显微镜新应用或高级显微镜发挥核心作用的工作。该期刊将发表关于显微镜和微分析的设计、方法、应用、实践或理论的文章，​​包括关于光学、电子束、X 射线显微断层扫描和扫描探针系统的报告。它还旨在定期发表评论论文、简短的交流以及关于显微镜和微分析当代发展的主题问题。该杂志包含原创研究，其中显微镜对生物学、生命科学、纳米科学和纳米技术、材料科学和工程的知识做出了重大贡献。

MICRON is an interdisciplinary forum suitable for all research involving new applications of microscopy or where microscopy plays a central role. The journal publishes articles on the design, methods, applications, practices, or theories of microscopy and microanalysis, including reports on optical microscopy, electron beam microscopy, X-ray microtomography, and scanning probe microscopy. It also aims to regularly publish commentary papers, brief communications, and topical issues on contemporary developments in microscopy and microanalysis. The journal features original research demonstrating significant contributions of microscopy to knowledge in biology, life sciences, nanoscience and nanotechnology, materials science, and engineering.

MICRON 是一个跨学科的论坛，适用于所有涉及显微镜新应用或显微镜在核心工作中发挥重要作用的研究。该期刊将发表关于显微镜和微分析的设计、方法、应用、实践或理论的文章，包括光学显微镜、电子束显微镜、X射线显微断层扫描和扫描探针显微镜的相关报告。它还旨在定期发表评论论文、简短交流以及关于显微镜和微分析当代发展的主题问题。该杂志包含原创研究，显微镜在生物学、生命科学、纳米科学与纳米技术、材料科学和工程等领域做出了重要贡献。

Micron

Image formation in structured illumination wide-field fluorescence microscopy.

It is shown that the optically sectioned images obtained with this approach possess the optical sectioning strengths comparable to those obtained with the confocal microscope, and that the transfer function behaviour is directly comparable to that of the true confocal instrument.

Category	Quartile
工程技术	3
工程技术, 显微镜技术	3

Image formation in structured illumination wide-field fluorescence microscopy.

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