Organ printing is a novel concept recently introduced in developing artificial three-dimensional organs to bridge the gap between transplantation needs and organ shortage. One of the major challenges is inclusion of blood-vessellike channels between layers to support cell viability, postprinting functionality in terms of nutrient transport, and waste removal. In this research, we developed a novel and effective method to print tubular channels encapsulating cells in alginate to mimic the natural vascular system. An experimental investigation into the influence on cartilage progenitor cell (CPCs) survival, and the function of printing parameters during and after the printing process were presented. CPC functionality was evaluated by checking tissue-specific genetic marker expression and extracellular matrix production. Our results demonstrated the capability of direct fabrication of cell-laden tubular channels by our newly designed coaxial nozzle assembly and revealed that the bioprinting process could induce quantifiable cell death due to changes in dispensing pressure, coaxial nozzle geometry, and biomaterial concentration. Cells were able to recover during incubation, as well as to undergo differentiation with high-level cartilage-associated gene expression. These findings may not only help optimize our system but also can be applied to biomanufacturing of 3D functional cellular tissue engineering constructs for various organ systems.

Yin Yu

Yahui Zhang

James A. Martin

Ibrahim T. Ozbolat

Journal of Biomechanical Engineering

Artificial Organs and Prostheses; Bioinstrumentation and Measurements; Bioheat Transfer; Biomaterials; Biomechanics; Bioprocess Engineering; Cellular Mechanics; Design and Control of Biological Systems; Physiological Systems.

The Journal of Biomechanical Engineering covers the following areas: artificial organs and prostheses, bioinstrumentation and measurements, bioheat transfer, biomaterials, biomechanics, bioprocess engineering, cellular mechanics, design and control of biological systems, and physiological systems.

《Journal of Biomechanical Engineering》期刊涵盖了以下领域：人工器官与假肢、生物仪器与测量、生物热传递、生物材料、生物力学、生物过程工程、细胞力学、生物系统的设计与控制以及生理系统。

Evaluation of Cell Viability and Functionality in Vessel-like Bioprintable Cell-Laden Tubular Channels

A novel and effective method to print tubular channels encapsulating cells in alginate to mimic the natural vascular system and revealed that the bioprinting process could induce quantifiable cell death due to changes in dispensing pressure, coaxial nozzle geometry, and biomaterial concentration.

Category	Quartile
医学	4
医学, 生物物理	3
医学, 工程生物医学	4

Evaluation of Cell Viability and Functionality in Vessel-like Bioprintable Cell-Laden Tubular Channels

ivySCI AI Smartly Parses PDF, Answers Researchers' Questions, and Helps You Understand Papers in Seconds

Journal Info

Category	Quartile
ENGINEERING, BIOMEDICAL	3