Rodent models constitute a cornerstone in the elucidation of the effects and biological mechanisms of 17β-estradiol. However, a thorough assessment of the methods for long-term administration of 17β-estradiol to mice is lacking. The fact that 17β-estradiol has been demonstrated to exert different effects depending on dose emphasizes the need for validated administration regimens. Therefore, 169 female C57BL/6 mice were ovariectomized and administered 17β-estradiol using one of the two commonly used subcutaneous methods; slow-release pellets (0.18 mg, 60-day release pellets; 0.72 mg, 90-day release pellets) and silastic capsules (with/without convalescence period, silastic laboratory tubing, inner/outer diameter: 1.575/3.175 mm, filled with a 14 mm column of 36 μg 17β-estradiol/mL sesame oil), or a novel peroral method (56 μg 17β-estradiol/day/kg body weight in the hazelnut cream Nutella). Forty animals were used as ovariectomized and intact controls. Serum samples were obtained weekly for five weeks and 17β-estradiol concentrations were measured using radioimmunoassay. The peroral method resulted in steady concentrations within--except on one occasion--the physiological range and the silastic capsules produced predominantly physiological concentrations, although exceeding the range by maximum a factor three during the first three weeks. The 0.18 mg pellet yielded initial concentrations an order of magnitude higher than the physiological range, which then decreased drastically, and the 0.72 mg pellet produced between 18 and 40 times higher concentrations than the physiological range during the entire experiment. The peroral method and silastic capsules described in this article constitute reliable modes of administration of 17β-estradiol, superior to the widely used commercial pellets.

E. Ingberg

A. Theodorsson

E. Theodorsson

J.O. Strom

General and Comparative Endocrinology

一般和比较内分泌学发表的文章涉及在亚分子、分子、细胞和有机体分析水平上脊椎动物和无脊椎动物内分泌系统的许多复杂性。

The journal "General and Comparative Endocrinology" publishes articles that explore the complexities of the endocrine systems of vertebrates and invertebrates at the submolecular, molecular, cellular, and organismal levels.

《General and Comparative Endocrinology》期刊发表的文章涉及脊椎动物和无脊椎动物内分泌系统在亚分子、分子、细胞和有机体分析水平上的复杂性。

Methods for long-term 17β-estradiol administration to mice

The peroral method and silastic capsules described in this article constitute reliable modes of administration of 17β-estradiol, superior to the widely used commercial pellets.

Category	Quartile
医学	3
医学, 内分泌学与代谢	4

Methods for long-term 17β-estradiol administration to mice

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