Abstract Transfer RNAs (tRNAs) are an essential component of the protein synthesis machinery. In order to accomplish their cellular functions, tRNAs go through a highly controlled biogenesis process leading to the production of correctly folded tRNAs. tRNAs in solution adopt the characteristic L-shape form, a stable tertiary conformation imperative for the cellular stability of tRNAs, their thermotolerance, their interaction with protein and RNA complexes and their activity in the translation process. The introduction of post-transcriptional modifications by modification enzymes, the global conformation of tRNAs, and their cellular stability are highly interconnected. We aim to further investigate this existing link by monitoring the maturation of bacterial tRNAs in E. coli extracts using NMR. Here, we report on the 1 H, 15 N chemical shift assignment of the imino groups and some amino groups of unmodified and modified E. coli tRNA Asp , tRNA Val and tRNA Phe , which are essential for characterizing their maturation process using NMR spectroscopy.

Marcel-Joseph Yared

Carine Chagneau

Pierre Barraud

Biomolecular NMR Assignments

BIOMOLECULAR NMR ASSIGNMENTS 提供了一个论坛，用于发布蛋白质和核酸的序列特异性共振分配作为分配说明。大分子中 NMR 活性核的化学位移包含有关分子构象和性质的详细信息。在 BIOMOLECULAR NMR ASSIGNMENTS 中发布共振分配可确保将这些数据存入 BIOMAGRESBANK (BMRB; HTTP://WWW.BMRB.WISC 的公共数据库中.EDU/)，可供其他研究人员使用。覆盖范围包括蛋白质和核酸；作业笔记被处理为快速在线发布，并在六月和十二月以半年一次的在线版本发布。

Biomolecular NMR Assignments provides a forum for publishing sequence-specific resonance assignments of proteins and nucleic acids. The chemical shifts of NMR-active nuclei in macromolecules contain detailed information about molecular conformation and properties. Resonance assignments published in Biomolecular NMR Assignments will be deposited in the public database BIOMAGRESBANK (BMRB; http://www.bmrb.wisc.edu/) for use by other researchers. The scope includes proteins and nucleic acids; assignment notes are processed for rapid online publication and released biannually in June and December.

《Biomolecular NMR Assignments》是一个专门发布蛋白质和核酸序列特异性共振分配的论坛。大分子中 NMR 活性核的化学位移提供了关于分子构象和性质的详细信息。在《Biomolecular NMR Assignments》上发布的共振分配数据将被存入公共数据库 BIOMAGRESBANK (BMRB; http://www.bmrb.wisc.edu/)，供其他研究人员使用。期刊涵盖蛋白质和核酸领域，作业笔记会被快速在线发布，并在每年的六月和十二月以半年一次的在线版本发布。

Category	Quartile
生物学	4
生物学, 生物物理	4
生物学, 光谱学	4

Imino chemical shift assignments of tRNAAsp, tRNAVal and tRNAPhe from Escherichia coli

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