Phytochelatins (PCs) exist widely in plants and are closely related to plant resistance to heavy metals (HMs).Glutathione (GSH) and nonprotein thiols (NPTs) are the major components of PCs.This study investigates the role of the PCs of Cladophora rupestris in Pb 2+ accumulation and detoxification.The distribution of Pb 2+ in the PCs of C. rupestris was studied.FTIR and XPS are used to characterize the chelating power of Pb 2+ with PCs in C. rupestris.The curve fitting of the secondary protein structure is used to identify the functional groups with Pb 2+ .Results showed that the content of Pb 2+ in the PCs of C. rupestris increased with an increase in Pb 2+ stress.Pb content increased to 352 and 314 mg/kg in NPTs and GSH, respectively, when Pb stress concentration reached 7.5 mg/dm 3 .The Pb 2+ fraction of C. rupestris PCs reached a maximum of 10.8 and 9.3% in NPTs and GSH, respectively.The Pb 2+ uptake by GSH and NPTs was 40-48% and 52-60%, respectively.Pb 2+ bound with the PCs of C. rupestris, forming complexes that contained Pb-OOC, Pb-C=O, CO-Pb, -N=Pb, Pb-NH2, Pb-O, Pb-N-, Pb-C-, Pb-S, and Pb 2+ with multiple groups of PCs as bridging ligand atoms.

None Lu-Sheng Zhang

None Liu Yang

None Lingsheng Li

None Xiaoyu Feng

None De-Ju Cao

None Zhao-Wen Liu

Environment Protection Engineering

Water purification, wastewater treatment, water reuse, solid waste disposal, gas emission abatement, systems of water and air pollution control, soil remediation.

The journal "Environmental Protection Engineering" focuses on research in water purification, wastewater treatment, water reuse, solid waste disposal, gas emission control, systems for water and air pollution control, and soil remediation.

《Environmental Protection Engineering》期刊专注于水净化、废水处理、水资源再利用、固体废物处置、气体排放控制以及水和空气污染控制系统的研究，同时涵盖土壤修复等相关领域。

Category	Quartile
环境科学与生态学	4
环境科学与生态学, 工程环境	4

Phytochelatins of Cladophora rupestris in Pb2+ absorption and its detoxification

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Journal Info

Category	Quartile
ENGINEERING, ENVIRONMENTAL	4