Grb10 (growth factor receptor-bound protein 10)-interacting GYF protein 1 (GIGYF1) can modulate insulin-like growth factor 1 receptor (IGF1R) signaling pathway, which plays an important role in regulating diabetes-associated cognitive impairment, by linking to Grb10 adapter. However, it remains unclear whether endogenous GIGYF1 expression is associated with the development of diabetes-related cognitive impairment. In this study, we measured the expression level of GIGYF1, Grb10, phosphorylated IGF1R/IGF1R, phosphorylated AKT serine/threonine protein kinase/protein kinase B (AKT)/AKT, and phosphorylated extracellular signal-regulated kinase (ERK)/ERK in human neuroblastoma SHSY-5Y cells. Meanwhile, we detected cell apoptosis, proliferation, and migration. Our results showed that the percentage of apoptotic cells increased along with the increasing concentrations of glucose (0-200 mM). The expression of GIGYF1 had a significant increase in the presence of 25 mM concentration of glucose in SHSY-5Y cells. In addition, high glucose augmented the expression of IGF1R and Grb10, but decreased the expression of p-IGF1R, p-AKT, and p-ERK. However, GIGYF1 knockdown reversed the decline in the expression of p-IGF1R, p-AKT, and p-ERK. In addition, knocking down GIGYF1 promoted the proliferation and migration of SHSY-5Y cells, but inhibited the apoptosis in SHSY-5Y cells. These results demonstrate that the expression of GIGYF1 can regulate IGF1R signaling pathway in high glucose-induced SHSY-5Y cells.

Ting Zhou

Yuefei Ma

Juan Tang

Fengqi Guo

Mingxia Dong

Qianping Wei

DNA and Cell Biology

DNA 和细胞生物学针对分子和细胞生物学的各个方面提供权威的、经过同行评审的研究，并专注于结合机制和临床研究来推动该领域的发展。DNA 和细胞生物学的覆盖范围包括：基因结构、功能和调控基因调控细胞激活的分子机制基因表达的转录、翻译或表观遗传控制机制分子医学分子发病机制癌症和自身免疫性疾病的遗传途径细胞和分子生物学的转化研究细胞器自噬细胞凋亡P 体过氧化物酶体蛋白质生物合成和降解蛋白质合成的调节翻译后修饰控制降解细胞自主炎症和宿主细胞反应对感染的反应对细胞因子和其他生理介质的病原体的逃避途径。

The journal "DNA and Cell Biology" focuses on various aspects of molecular and cell biology, providing authoritative, peer-reviewed research. It aims to integrate mechanisms with clinical studies to advance the field. The scope includes: the structure, function, and regulation of genes; molecular mechanisms of gene regulation and cell activation; transcriptional, translational, or epigenetic control mechanisms of gene expression; molecular medicine; molecular pathogenesis; genetic pathways of cancer and autoimmune diseases; translational research in cell and molecular biology; autophagy and apoptosis of organelles; P bodies and peroxisomes; protein biosynthesis and degradation; regulation of protein synthesis; post-translational modifications; cellular degradation; autonomous inflammation and host cell responses to infection; and pathogen evasion pathways against cytokines and other physiological mediators.

《DNA and Cell Biology》期刊专注于分子和细胞生物学的各个方面，提供权威的、经过同行评审的研究。该期刊致力于结合机制与临床研究，推动该领域的发展。其覆盖范围包括：基因的结构、功能和调控；基因调控细胞激活的分子机制；基因表达的转录、翻译或表观遗传控制机制；分子医学；分子发病机制；癌症和自身免疫性疾病的遗传途径；细胞和分子生物学的转化研究；细胞器的自噬与凋亡；P体和过氧化物酶体；蛋白质的生物合成与降解；蛋白质合成的调节；翻译后修饰；细胞的降解；自主炎症及宿主细胞对感染的反应；以及对细胞因子和其他生理介质的病原体逃避途径。

Modulation of<i>IGF1R</i>Signaling Pathway by<i>GIGYF1</i>in High Glucose-Induced SHSY-5Y Cells

It is demonstrated that the expression of GIGYF1 can regulate IGF1R signaling pathway in high glucose-induced SHSY-5Y cells and knockdown reversed the decline in theexpression of p-IGF1R, p-AKT, and p-ERK.

Category	Quartile
生物学	4
生物学, 生化与分子生物学	4
生物学, 细胞生物学	4
生物学, 遗传学	4

Modulation ofIGF1RSignaling Pathway byGIGYF1in High Glucose-Induced SHSY-5Y Cells

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Journal Info

Category	Quartile
GENETICS & HEREDITY	2