A dual-wavelength excitation Raman probe with laser inputs at 866 nm or 1064 nm is customized and integrated into a compact Raman spectrometer that is based on an InGaAs detector. Under 1064 nm illumination, the spectrometer detects fingerprint Raman signals below 2000 cm –1 . While under 866 nm illumination, the spectral range is extended to cover high-frequency region (2400–4000 cm –1 ) that includes major C–H and O–H Raman vibrations. We demonstrate that the dual excitation InGaAs Raman is beneficial in detecting high-frequency Raman signals, especially water contents in high-fluorescent biological samples such as human dental tissues, grape skin, and plum skin due to the suppressed fluorescence interference.

Wencai He

Bolan Li

Shan Yang

Applied Spectroscopy

APPLIED SPECTROSCOPY 是世界领先的光谱学期刊之一，发表高质量的同行评议文章，包括基础和应用，涵盖光谱学的各个方面。该杂志成立于 1951 年，由应用光谱学学会拥有并出版该杂志致力于履行协会的使命，即“……推进和传播有关光谱学和其他相关科学的艺术和科学的知识和信息。”

Applied Spectroscopy is one of the world's leading journals in spectroscopy, publishing high-quality peer-reviewed articles that cover both fundamental and applied aspects of spectroscopy. Established in 1951, the journal is owned and published by the Society for Applied Spectroscopy, dedicated to advancing and disseminating knowledge and information related to the art and science of spectroscopy and other related sciences.

《Applied Spectroscopy》是全球领先的光谱学期刊之一，发表高质量的同行评审文章，涵盖光谱学的基础和应用各个方面。该杂志成立于1951年，由应用光谱学学会拥有并出版，致力于推进和传播光谱学及其他相关科学的知识与信息。

High-Frequency Raman Analysis in Biological Tissues Using Dual-Wavelength Excitation Raman Spectroscopy

It is demonstrated that the dual excitation InGaAs Raman is beneficial in detecting high-frequency Raman signals, especially water contents in high-fluorescent biological samples such as human dental tissues, grape skin, and plum skin due to the suppressed fluorescence interference.

Category	Quartile
化学	3
化学, 光谱学	3
化学, 仪器仪表	4

High-Frequency Raman Analysis in Biological Tissues Using Dual-Wavelength Excitation Raman Spectroscopy

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