Brassinosteroids (BR) are multifunctional plant hormones that can regulate growth and development and respond to abiotic stresses. In this study, we investigated the effect of one of brassinosteroids -24-epibrassinolide (EBL) in alleviating of cold tolerance in winter turnip rape. The results showed that EBL pretreatment could reduce the degree of membrane damage by lipid peroxidation, alleviate ROS burst, improve the osmotic potential, stomatal conductance, photosynthetic efficiency, and antioxidant capacity of winter turnip rape under cold treatment. Compared with EBL + 4 °C treatment, EBL + LaCl 3 + 4 °C and EBL + EGTA + 4 °C treatments can significantly increase the degree of membrane lipid damage, reactive oxygen species (ROS) content, decrease the osmotic adjustment and antioxidation ability, reduce photosynthetic efficiency and stomatal conductance in winter turnip rape. RNA-Seq analysis showed that there were 116 differentially expressed genes (DEGs) in EL (EBL + 4 °C) vs. L (4 °C), including 70 up-regulated genes and 46 down-regulated genes. These DEGs play a critical role in regulating various physiological processes in plants, such as photosynthesis, osmotic regulation of biofilm systems, Ca 2+ signal transduction, redox reactions. We selected the 10 DEGs for further qRT-PCR verification and the results were correlated with the results of RNA-sEq. In conclusion, our research demonstrates that BRs play an important role in enhancing cold tolerance of winter turnip rape. Moreover, additionally LaCl 3 and EGTA effects was studied and discussed together with EBL, the results show that Ca 2+ signalling is involved in BRs-induced cold tolerance.

Yao Li

Yuanpei Sun

Cheng Ma

Xin Kang

Juan Wang

Tengguo Zhang

Biologia

BIOLOGIA 成立于 1946 年，发表微生物、植物和动物科学领域的高质量研究论文。微生物科学论文涵盖细菌、古细菌和微生物真核生物的所有方面，包括生物化学、细胞和分子生物学、基因组学、蛋白质组学和生物信息学。植物科学主题包括分类学、地球植物学、遗传学和实验植物学的所有领域的基础研究，包括细胞、全植物和群落生理学。动物学涵盖从细胞到分子水平的动物系统学和分类学、形态学、生态学和生理学。

The journal BIOLOGIA was established in 1946 and publishes high-quality research papers in the fields of microbiology, plant science, and animal science. Papers in microbiology cover all aspects of bacteria, archaea, and eukaryotic microorganisms, including biochemistry, cell and molecular biology, genomics, proteomics, and bioinformatics. The topics in plant science encompass fundamental research in taxonomy, earth botany, genetics, and experimental botany, covering areas such as cellular, whole plant, and community physiology. Zoology includes studies on animal systematics and taxonomy, morphology, ecology, and physiology at both cellular and molecular levels.

BIOLOGIA 期刊成立于1946年，专注于微生物、植物和动物科学领域的高质量研究论文。微生物科学的论文涵盖细菌、古细菌和真核微生物的各个方面，包括生物化学、细胞和分子生物学、基因组学、蛋白质组学以及生物信息学。植物科学的主题包括分类学、地球植物学、遗传学和实验植物学的基础研究，涉及细胞、全植物和群落生理学等领域。动物学研究则涵盖从细胞到分子水平的动物系统学和分类学、形态学、生态学和生理学等内容。

Category	Quartile
生物学	4
生物学, 生物学	4

24-epibrassinolide enhanced cold tolerance of winter turnip rape (Brassica rapa L.)

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