A tris (2-carboxyethyl) phosphine (TCEP) related cleavage on cysteine-containing proteins

Peiran Liu, Brian W. O’Mara, Bethanne M. Warrack, Wei Wu, Yunping Huang, Yihong Zhang, Rulin Zhao, Mei Lin, Michael S. Ackerman, Peter K. Hocknell, Guodong Chen, Li Tao, Siegfried Rieble, Jack Wang, David B. Wang-Iverson, Adrienne A. Tymiak, Michael J. Grace, Reb J. Russell

DOI: 10.1016/j.jasms.2010.01.016

Journal: Journal of the American Society for Mass Spectrometry

The observation of a side reaction that cleaves protein backbones under mild conditions by fracturing the Cysteine residues, thus generating heterogeneous peptides containing different moieties from the fractured cysteine.