Using a monoclonal antibody based ELISA, 600 pAN7-1 plasmid-tagged mutants of Penicillium paxilli were screened for paxilline accumulation and one paxilline-negative mutant, YI-20, was identified. A molecular analysis of this mutant showed that pAN7-1 was inserted at a single site but was present as 4-6 copies arranged in a head-to-tail tandem array. Rescue of flanking sequences and analysis of the corresponding genomic region revealed that YI-20 has an extensive deletion at the site of pAN7-1 integration. Probing of a CHEF gel with the same sequences showed that associated with the deletion is a rearrangement of chromosome Va. Targeted gene disruption of wild-type sequences adjacent to the site where pAN7-1 inserted, resulted in the generation of two additional paxilline-negative mutants; both were single crossovers with deletions extending outside the region mapped. Neither of these new mutants had a rearrangement of chromosome Va, suggesting that deletion of genes on this chromosome is responsible for the paxilline-negative phenotype. Telomeric fingerprinting of genomic digests of P. paxilli, combined with pulsed-field gel electrophoresis of chromosomal DNA, established that there are a minimum of eight chromosomes in this fungus.

C Young

Y Itoh

R Johnson

I Garthwaite

C O Miles

S C Munday-Finch

B Scott

Current genetics

CURRENT GENETICS 发表真核和原核微生物和细胞器的遗传、基因组、分子和系统级分析。所有文章均经过同行评审。该杂志欢迎采用任何类型的研究方法提交，无论是分析（旨在更好地理解）、应用（旨在实际应用）、合成或理论。CURRENT GENETICS 不再接受描述线粒体/叶绿体基因组序列的手稿少数种类。仍将考虑涵盖包含大量物种的序列比较和分析的手稿。

CURRENT GENETICS publishes genetic, genomic, molecular, and systems-level analyses of eukaryotic and prokaryotic microorganisms and organelles. All articles undergo peer review. The journal welcomes submissions employing any type of research methodology, whether analytical (aimed at better understanding), applied (aimed at practical application), synthetic, or theoretical. CURRENT GENETICS no longer accepts manuscripts that solely describe mitochondrial or chloroplast genome sequences, but will still consider manuscripts covering sequence comparisons and analyses involving a large number of species.

CURRENT GENETICS 发表关于真核生物和原核生物及其细胞器的遗传、基因组、分子和系统级分析。所有文章均经过同行评审。该杂志欢迎采用各种研究方法的投稿，无论是分析性研究（旨在深入理解）、应用性研究（旨在实际应用）、综合性研究还是理论研究。CURRENT GENETICS 不再接受仅描述线粒体或叶绿体基因组序列的手稿，但仍会考虑涉及大量物种的序列比较和分析的手稿。

Category	Quartile
生物学	4
生物学, 遗传学	4

Paxilline-negative mutants of Penicillium paxilli generated by heterologous and homologous plasmid integration.

ivySCI AI Smartly Parses PDF, Answers Researchers' Questions, and Helps You Understand Papers in Seconds

Journal Info