In the clustered regulatory interspaced short palindromic repeats (CRISPR)/CRISPR associated protein (Cas) system, protoplasts are not only useful for rapidly validating the mutagenesis efficiency of various RNA-guided endonucleases, promoters, sgRNA designs, or Cas proteins, but can also be a platform for DNA-free gene editing. To date, the latter approach has been applied to numerous crops, particularly those with complex genomes, a long juvenile period, a tendency for heterosis, and/or self-incompatibility. Protoplast regeneration is thus a key step in DNA-free gene editing. In this report, we review the history and some future prospects for protoplast technology, including protoplast transfection, transformation, fusion, regeneration, and current protoplast applications in CRISPR/Cas-based breeding.

Jin-Jun Yue

Jin-Ling Yuan

Fu-Hui Wu

Yu-Hsuan Yuan

Qiao-Wei Cheng

Chen-Tran Hsu

Choun-Sea Lin

Frontiers in Genome Editing

Protoplasts: From Isolation to CRISPR/Cas Genome Editing Application

The history and some future prospects for protoplast technology, including protoplasts transfection, transformation, fusion, regeneration, and current protoplast applications in CRISPR/Cas-based breeding are reviewed.

Protoplasts: From Isolation to CRISPR/Cas Genome Editing Application

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Journal Info

Category	Quartile
GENETICS & HEREDITY	1