Abstract Objective Invasions of nonnative fish species, such as Brook Trout Salvelinus fontinalis , disrupt ecosystem function and adversely impact native populations through competition, hybridization, and predation. Timely detection of invasive species is crucial for effective management and prevention of further spread. Environmental DNA (eDNA) technology is a powerful tool for monitoring the spread of invasive species, especially in aquatic environments, where direct observation is challenging. While eDNA sampling is sensitive and cost‐effective, real‐time analysis remains a challenge. Methods To address this limitation, we developed eDNA detection tools, using CRISPR‐Cas12a technology, that facilitate the rapid identification of invasive Brook Trout and native cutthroat trout (Lahontan Cutthroat Trout Oncorhynchus henshawi , Westslope Cutthroat Trout O. lewisi , Rocky Mountain Cutthroat Trout O. virginalis , or Coastal Cutthroat Trout O. clarkii ), without the need for specialized laboratory equipment. We employed this technology in Marsh Creek, a semi‐isolated tributary to the Sultan River in Washington State with no previous documentation of invasive Brook Trout. Result We successfully identified Brook Trout and cutthroat trout eDNA during on‐site analysis at Marsh Creek and found that the sensitivity of detecting these species can be increased with improvements in eDNA purification technology. Our results identified that Brook Trout were present in the lower reaches of the stream, particularly in areas of slow‐moving water. Conclusion The portability and simplicity of this method offer the potential for streamside eDNA detection of invasive species, and it could become another tool in the fight against the spread of invasive Brook Trout in the western United States.

Tholen J. Blasko

Samantha Larson

Michael P. Phelps

Transactions of the American Fisheries Society

TRANSACTIONS OF THE AMERICAN FISHERIES SOCIETY 是一份备受推崇的国际渔业科学期刊，自 1872 年以来一直持续出版。它收录了遗传学、生理学、生物学、生态学、人口动态、经济学、健康、文化和其他主题与海洋和淡水鱼类和贝类及其各自的渔业和环境密切相关。

Transactions of the American Fisheries Society is a prestigious international journal in the field of fisheries science, continuously published since 1872. It includes high-quality research covering genetics, physiology, biology, ecology, population dynamics, economics, health, culture, and other topics closely related to marine and freshwater fish and shellfish, as well as their respective fisheries and environments.

《Transactions of the American Fisheries Society》是一本享有盛誉的国际渔业科学期刊，自1872年创刊以来，持续发表高质量的研究成果。期刊涵盖遗传学、生理学、生物学、生态学、种群动态、经济学、健康、文化等多个领域，涉及海洋和淡水鱼类及其渔业和环境的相关主题。

Category	Quartile
农林科学	3
农林科学, 渔业	3

Rapid identification of invasive Brook Trout through CRISPR‐based environmental DNA detection

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