The resistance of the emerging human pathogen Stenotrophomonas maltophilia to tetracycline antibiotics mainly depends on multidrug efflux pumps and ribosomal protection enzymes. However, the genomes of several strains of this Gram-negative bacterium code for a FAD-dependent monooxygenase (SmTetX) homologous to tetracycline destructases. This protein was recombinantly produced and its structure and function were investigated. Activity assays using SmTetX showed its ability to modify oxytetracycline with a catalytic rate comparable to those of other destructases. SmTetX shares its fold with the tetracycline destructase TetX from Bacteroides thetaiotaomicron; however, its active site possesses an aromatic region that is unique in this enzyme family. A docking study confirmed tetracycline and its analogues to be the preferred binders amongst various classes of antibiotics.

Martin Malý

Petr Kolenko

Jan Stránský

Leona Švecová

Jarmila Dušková

Tomáš Koval'

Tereza Skálová

Mária Trundová

Kristýna Adámková

Jiří Černý

Paulína Božíková

Jan Dohnálek

Acta Crystallographica Section F Structural Biology Communications

ACTA CRYSTALLOGRAPHICA SECTION F 是一本快速结构生物学通讯期刊。该期刊欢迎有关结构生物学任何方面的文章，包括使用高通量方法确定的结构或来自诸如制药行业使用的迭代研究的结构。该期刊提供开放访问选项和所有通信都受益于无限制免费使用彩色插图且不收取页面费用。鼓励作者提交多媒体内容以与他们的文章一起发表。ACTA CRYST。 F 有一个名为 PUBLBIO 的专用在线工具，旨在使作者更轻松地准备和提交文章。

Acta Crystallographica Section F: Structural Biology Communications is a rapid communications journal dedicated to structural biology research. The journal welcomes articles on all aspects of structural biology, including structures determined using high-throughput methods or those derived from iterative studies in the pharmaceutical industry. It offers open access options, and all communications benefit from unlimited free use of color illustrations without page charges. Authors are encouraged to submit multimedia content to accompany their articles. Additionally, Acta Crystallographica Section F provides a dedicated online tool called PUBLBIO, designed to assist authors in preparing and submitting their manuscripts more easily.

《Acta Crystallographica Section F: Structural Biology Communications》是一本快速发表结构生物学研究成果的通讯期刊。该期刊欢迎关于结构生物学各个方面的文章，包括使用高通量方法获得的结构，或来自制药行业的迭代研究所产生的结构。期刊提供开放获取选项，所有通信均可无限制免费使用彩色插图，且不收取页面费用。我们鼓励作者提交多媒体内容以与文章一同发表。此外，《Acta Crystallographica Section F》还提供名为PUBLBIO的专用在线工具，旨在帮助作者更轻松地准备和提交文章。

Acta Crystallographica Section F:Structural Biology Communications

Tetracycline-modifying enzymeSmTetX fromStenotrophomonas maltophilia

来自嗜麦芽窄食单胞菌的四环素修饰酶SmTetX

Category	Quartile
生物学	4
生物学, 生化研究方法	4
生物学, 生化与分子生物学	4
生物学, 生物物理	4
生物学, 晶体学	4

Tetracycline-modifying enzymeSmTetX fromStenotrophomonas maltophilia

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