Chronic dacryocystitis (CD) can result in severe complications and vision impairment due to ongoing microbial infections and persistent tearing. Tear fluid, which contains essential components vital for maintaining ocular surface health, has been investigated for its potential in the noninvasive identification of ocular biomarkers through metabolomics analysis. In this study, we employed UHPLC-MS/MS to analyze the tear metabolome of CD patients. UHPLC-MS/MS analysis of tear samples from CD patients revealed significant metabolic alterations. Compared with the control group, 298 metabolites were elevated, while 142 were decreased. KEGG pathway analysis suggested that these changes primarily affected arginine and proline metabolism, biosynthesis of amino acids, and phenylalanine biosynthesis in CD. Notably, 3-dehydroquinic acid, anthranilic acid, citric acid, and l-isoleucine emerged as potential biomarker candidates of CD with high diagnostic accuracy (AUC = 0.94). These findings suggest that tear fluid metabolism, particularly amino acid biosynthesis, plays a significant role in the pathogenesis of CD. Uncovering these metabolic products and pathways provides valuable insights into the mechanisms underlying CD and paves the way for the development of diagnostic tools and targeted therapies.

Shuai Ben

Qun Zheng

Ya Zhao

Jiao Xia

Wan Mu

Mudi Yao

Biao Yan

Qin Jiang

Journal of Proteome Research

《蛋白质组研究杂志》出版内容涵盖全球蛋白质分析和功能的各个方面，包括基因组学、时空蛋白质组学、代谢组学和代谢组学、临床和农业蛋白质组学的动态方面，以及包括生物信息学在内的方法学进展。主题和重点是通过不同类型的“组学”之间的协同作用，对生命科学采取多学科方法。

The Journal of Proteome Research covers various aspects of global protein analysis and functionality, including genomics, spatiotemporal proteomics, metabolomics, and dynamic studies in clinical and agricultural proteomics, as well as methodological advancements including bioinformatics. The journal focuses on a multidisciplinary approach to life sciences through the synergy of different types of 'omics'.

《Journal of Proteome Research》涵盖全球蛋白质分析和功能的各个方面，包括基因组学、时空蛋白质组学、代谢组学、临床和农业蛋白质组学的动态研究，以及生物信息学等方法学的进展。该期刊的主题和重点是通过不同类型的“组学”之间的协同作用，采用多学科的方法来研究生命科学。

Category	Quartile
生物学	2
生物学, 生化研究方法	2

Tear Fluid-Based Metabolomics Profiling in Chronic Dacryocystitis Patients

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Journal Info

Category	Quartile
BIOCHEMICAL RESEARCH METHODS	1