Yellow fever virus (YFV) circulates in a sylvatic cycle between non-human primates (NHPs) and arboreal mosquitoes in Brazil. Passive monitoring of ill or deceased NHPs is a key component of the Brazilian yellow fever (YF) surveillance program. Samples from NHPs carcasses are usually suitable for molecular tests but not for serological assays. As an alternative to the conventional plaque reduction neutralization test (PRNT) based on sera, we tested the utility of liver homogenates from experimentally infected (YFV, Mayaro virus [MAYV], chikungunya virus [CHIKV], or mock) mice to quantify PRNTs. Although homogenates from mock-infected mice showed a low level of nonspecific virus neutralization against all three viruses, homogenates from YFV-, MAYV- and CHIKV-infected mice demonstrated significantly higher levels of virus neutralization compared to controls. Receiver operating characteristic (ROC) curves analyses were performed using the median neutralization values of three technical replicates for each infected group separately or collectively. Results showed scores ≥0.97 (95% CI ≥ 0.89–1.0) for the area under the curve at dilutions 1:20 to 1:80, suggesting that median virus neutralization values effectively differentiated infected mice from controls. Liver homogenates obtained from 25 NHP carcasses (collected during the 2017 YF outbreak in Brazil) were also tested using the adapted PRNT as well as rapid lateral flow tests to investigate anti-YFV IgM. Neutralization activity was detected in six NHP samples that were also positive by PCR and anti-YFV IgM tests and one sample that tested negative by PCR and IgM test. Our results demonstrate the feasibility of using liver homogenates as an alternative approach for serological investigation in viral epidemiologic surveillance.

Thaís Alkifeles Costa

Matheus Soares Arruda

Gabriela Fernanda Garcia Oliveira

Erik Vinicius de Sousa Reis

Anna Catarina Dias Soares Guimarães

Gabriel Dias Moreira

Nidia Esther Colquehuanca Arias

Marina do Vale Beirão

Nikos Vasilakis

Kathryn A. Hanley

Betânia Paiva Drumond

PLoS neglected tropical diseases

PLOS 被忽视的热带病发表的研究致力于被忽视的热带病（NTDS）的病理学、流行病学、预防、治疗和控制以及相关的公共政策。NTDS 被定义为一组促进贫困的慢性传染病，它主要发生在低收入和中等收入国家的农村地区和城市贫困地区。它们对儿童健康和发育、怀孕和工人生产力的影响，以及它们的污名化特征限制了经济稳定性。考虑到这些疾病的所有方面，包括：发病机制临床特征药理学和治疗诊断流行病学矢量生物学疫苗学和预防人口、生态和社会决定因素公共卫生和政策方面（包括成本效益分析）。

The journal PLoS Neglected Tropical Diseases publishes research focused on the pathology, epidemiology, prevention, treatment, and control of neglected tropical diseases (NTDs), as well as related public policies. NTDs are defined as a group of chronic infectious diseases that contribute to poverty, primarily occurring in rural areas and urban slums of low- and middle-income countries. These diseases impact child health and development, pregnancy, and worker productivity, while their stigmatizing characteristics hinder economic stability. The journal addresses all aspects of these diseases, including: pathogenesis, clinical features, pharmacology and treatment, diagnosis, epidemiology, vector biology, vaccinology and prevention, population, ecological and social determinants, and public health and policy issues (including cost-effectiveness analysis).

《PLoS Neglected Tropical Diseases》期刊发表的研究专注于被忽视的热带病（NTDs）的病理学、流行病学、预防、治疗和控制，以及相关的公共政策。NTDs 被定义为一组促进贫困的慢性传染病，主要发生在低收入和中等收入国家的农村和城市贫困地区。这些疾病对儿童健康和发育、孕妇及工人的生产力产生影响，同时其污名化特征也限制了经济的稳定性。期刊考虑到这些疾病的各个方面，包括：发病机制、临床特征、药理学与治疗、诊断、流行病学、媒介生物学、疫苗学与预防、人口、生态和社会决定因素，以及公共卫生和政策方面（包括成本效益分析）。

Category	Quartile
医学	2
医学, 热带医学	1
医学, 寄生虫学	2

Detection of neutralizing antibodies against arboviruses from liver homogenates

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Journal Info

Category	Quartile
TROPICAL MEDICINE	1