The interlamellar matrix (ILM), located between the annular layers of the intervertebral disc, is an adhesive component which acts to resist delamination. Investigating the mechanical properties of the ILM can provide us with valuable information regarding risk of disc injury; however given its viscoelastic nature, it may be necessary to conduct preconditioning on tissue samples before measuring these ILM properties. Therefore, the aim of this study was to optimize mechanical testing protocols of the ILM by examining the effect of preconditioning on stiffness and strength of this adhesive matrix. Eighty-eight annular samples were dissected from twenty-two porcine cervical discs and randomized into one of four testing conditions consisting of 10 cycles of 15% strain followed by a 180° adhesive peel test. The four testing groups employed a different strain rate for the 10 cycles of preconditioning: 0.01 mm/s (n=23); 0.1 mm/s (n=26); 1 mm/s (n=23); no preconditioning employed (n=16). Samples preconditioned at 0.01 mm/s were significantly less stiff than those that had not received preconditioning (p=0.014). No other results were found to be statistically significant. Given the lack of differences observed in this study, preconditioning is likely not necessary prior to conducting a 180° peel test. However, if preconditioning is employed, the findings from this study suggest avoiding preconditioning conducted at very slow rates (i.e. 0.01 mm/s) as the long testing time may negatively affect the tissue.

Sabrina I. Sinopoli

Mitchel Whittal

K. Josh Briar

Diane E. Gregory

Journal of Biomechanical Engineering

Artificial Organs and Prostheses; Bioinstrumentation and Measurements; Bioheat Transfer; Biomaterials; Biomechanics; Bioprocess Engineering; Cellular Mechanics; Design and Control of Biological Systems; Physiological Systems.

The Journal of Biomechanical Engineering covers the following areas: artificial organs and prostheses, bioinstrumentation and measurements, bioheat transfer, biomaterials, biomechanics, bioprocess engineering, cellular mechanics, design and control of biological systems, and physiological systems.

《Journal of Biomechanical Engineering》期刊涵盖了以下领域：人工器官与假肢、生物仪器与测量、生物热传递、生物材料、生物力学、生物过程工程、细胞力学、生物系统的设计与控制以及生理系统。

Category	Quartile
医学	4
医学, 生物物理	3
医学, 工程生物医学	4

Does annulus fibrosus lamellar adhesion testing require preconditioning?

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Journal Info

Category	Quartile
ENGINEERING, BIOMEDICAL	3