Cytochrome P450 (P450) enzymes account for ~75% of the metabolism of drugs. Most of the reactions catalyzed by P450s are mixed‐function oxidations, and a C–H bond is (usually) broken. The rate‐limiting nature of this step can be analyzed using the kinetic isotope effect (KIE) approach. The most relevant type of KIE is one termed intermolecular non‐competitive, indicative of rate‐limiting C–H bond breaking. A plot of KIE versus k cat for several P450s showed a correlation coefficient ( r 2 ) of 0.62. Deuterium substitution has been considered as a potential means of slowing drug metabolism or redirecting sites of metabolism in some cases, and several general points can be made regarding the potential for application of deuterium in drug design/development based on what is known about P450 KIEs. Copyright © 2013 John Wiley &amp; Sons, Ltd.

F. Peter Guengerich

Journal of Labelled Compounds and Radiopharmaceuticals

《标记化合物和放射性药物杂志》发表了涉及标记化合物制备和这些化合物应用的研究的所有方面。这包括用于体外和体内医学、药理学、生物学、生化和化学研究的示踪方法。《标记化合物和放射性药物杂志》特别关注放射性药物的生物医学研究、诊断和治疗应用，涵盖从基础到发展的所有阶段基于物理和化学特征的分子结构、配位化合物和纳米粒子的代谢研究和技术开发到临床前和临床研究。

The Journal of Labelled Compounds and Radiopharmaceuticals publishes research covering all aspects of the preparation and application of labelled compounds. This includes tracing methods used in in vitro and in vivo medicine, pharmacology, biology, biochemistry, and chemical research. The journal places special emphasis on biomedical research involving radiopharmaceuticals, as well as their diagnostic and therapeutic applications, covering all stages from fundamental studies to preclinical and clinical research, focusing on the metabolic studies and technological developments of molecular structures, coordination compounds, and nanoparticles based on their physical and chemical characteristics.

《Journal of Labelled Compounds and Radiopharmaceuticals》发表涉及标记化合物制备及其应用的研究，涵盖所有相关领域。这些领域包括体外和体内医学、药理学、生物学、生化和化学研究中的示踪方法。该期刊特别关注放射性药物的生物医学研究及其在诊断和治疗中的应用，涵盖从基础研究到临床前和临床研究的各个阶段，涉及基于物理和化学特征的分子结构、配位化合物和纳米粒子的代谢研究与技术开发。

Kinetic deuterium isotope effects in cytochrome P450 oxidation reactions

Several general points can be made regarding the potential for application of deuterium in drug design/development based on what is known about P450 KIEs.

Category	Quartile
医学	4
医学, 生化研究方法	4
医学, 分析化学	4
医学, 药物化学	4

Kinetic deuterium isotope effects in cytochrome P450 oxidation reactions

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Category	Quartile
CHEMISTRY, MEDICINAL	4