Natural macrocyclic peptides produced by microorganisms serve as valuable resources for therapeutic compounds, including antibiotics, anticancer agents, and immune suppressive agents. Nonribosomal peptide synthetases (NRPSs) are responsible for the biosynthesis of macrocyclic peptides. NRPSs are large multimodular enzymes, and each module recognizes and incorporates one specific amino acid into the polypeptide product. In the final biosynthetic step, the mature linear peptide precursor is subject to head-to-tail cyclization by the thioesterase (TE) domain in the C-terminal module. Since the TE domains can autonomously catalyze the cyclization of diverse linear peptide substrates, isolated TE domains can be used to produce natural product derivatives. To understand the mechanism of TE domains in NRPSs as a base for therapeutic applications, we investigated the TE domain (residues 6236-6486) of tyrocidine synthetase TycC by NMR. Tyrocidine is a cyclic decapeptide with antibiotic activity, and TycC-TE catalyzes the cyclization of the linear decapeptide precursor. Here, we report the backbone resonance assignments of TycC-TE. The assignments of TycC-TE provide the basis for NMR investigations of the structure and substrate-recognition mode of the TE domain in NRPS.

Mitsuhiro Takeda

Rino Saito

Sho Konno

Takayuki Nagae

Hiroshi Aoyama

Sosuke Yoshinaga

Hiroaki Terasawa

Akihiro Taguchi

Atsuhiko Taniguchi

Yoshio Hayashi

Masaki Mishima

Biomolecular NMR Assignments

BIOMOLECULAR NMR ASSIGNMENTS 提供了一个论坛，用于发布蛋白质和核酸的序列特异性共振分配作为分配说明。大分子中 NMR 活性核的化学位移包含有关分子构象和性质的详细信息。在 BIOMOLECULAR NMR ASSIGNMENTS 中发布共振分配可确保将这些数据存入 BIOMAGRESBANK (BMRB; HTTP://WWW.BMRB.WISC 的公共数据库中.EDU/)，可供其他研究人员使用。覆盖范围包括蛋白质和核酸；作业笔记被处理为快速在线发布，并在六月和十二月以半年一次的在线版本发布。

Biomolecular NMR Assignments provides a forum for publishing sequence-specific resonance assignments of proteins and nucleic acids. The chemical shifts of NMR-active nuclei in macromolecules contain detailed information about molecular conformation and properties. Resonance assignments published in Biomolecular NMR Assignments will be deposited in the public database BIOMAGRESBANK (BMRB; http://www.bmrb.wisc.edu/) for use by other researchers. The scope includes proteins and nucleic acids; assignment notes are processed for rapid online publication and released biannually in June and December.

《Biomolecular NMR Assignments》是一个专门发布蛋白质和核酸序列特异性共振分配的论坛。大分子中 NMR 活性核的化学位移提供了关于分子构象和性质的详细信息。在《Biomolecular NMR Assignments》上发布的共振分配数据将被存入公共数据库 BIOMAGRESBANK (BMRB; http://www.bmrb.wisc.edu/)，供其他研究人员使用。期刊涵盖蛋白质和核酸领域，作业笔记会被快速在线发布，并在每年的六月和十二月以半年一次的在线版本发布。

Category	Quartile
生物学	4
生物学, 生物物理	4
生物学, 光谱学	4

Backbone resonance assignments of the C-terminal thioesterase domain of tyrocidine synthetase C

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