Membrane proteins are a large, diverse group of proteins, serving a multitude of cellular functions. They are difficult to study because of their requirement of a lipid membrane for function. Here we show that two-photon polarization microscopy can take advantage of the cell membrane requirement to yield insights into membrane protein structure and function, in living cells and organisms. The technique allows sensitive imaging of G-protein activation, changes in intracellular calcium concentration and other processes, and is not limited to membrane proteins. Conveniently, many suitable probes for two-photon polarization microscopy already exist.

Josef Lazar

Alexey Bondar

Stepan Timr

Stuart J Firestein

Nature methods

NATURE METHODS 是一个发表新方法和显着改进生命科学中久经考验的基础研究技术的论坛。该月刊面向广泛的跨学科受众，包括积极参与实验室实践的学术和行业研究人员。它为他们提供了进行研究的新工具，并强调所提出的工作的直接实际相关性。该杂志发表主要研究论文以及最近技术和方法发展的概述。我们正在积极寻找与生物和生物医学科学相关的主要方法论文，包括以化学为基础的方法，这些方法在生物学问题的研究中具有实际应用。

NATURE METHODS is a journal dedicated to publishing new methods and significant improvements in established research techniques within the life sciences. This monthly publication targets a broad interdisciplinary audience, including academic and industry researchers actively engaged in laboratory practices. It provides researchers with new tools for conducting studies and emphasizes the direct practical relevance of the work presented. The journal publishes primary research articles as well as reviews on recent developments in technologies and methods. We are actively seeking major method papers related to biological and biomedical sciences, particularly those based on chemistry that have practical applications in the study of biological questions.

NATURE METHODS 是一个专注于发表生命科学领域新方法和显著改进的基础研究技术的期刊。该月刊面向广泛的跨学科受众，包括积极参与实验室实践的学术和行业研究人员。它为研究人员提供了新的研究工具，并强调所提出工作的直接实际相关性。该杂志发表主要研究论文以及最近技术和方法发展的综述。我们积极寻找与生物和生物医学科学相关的主要方法论文，特别是那些在生物学研究中具有实际应用的化学基础方法。

Nature Methods

Nat Methods

Two-photon polarization microscopy reveals protein structure and function.

It is shown that two-photon polarization microscopy can take advantage of the cell membrane requirement to yield insights into membrane protein structure and function, in living cells and organisms.

Category	Quartile
生物学	1
生物学, 生化研究方法	1

Two-photon polarization microscopy reveals protein structure and function.

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