Skin disorders pose a significant health burden globally, affecting millions of individuals across diverse demographics. Advancements in molecular techniques have revolutionised our understanding of the underlying mechanisms of skin disorders, offering insights into their pathogenesis, diagnosis, and potential targeted treatment. Furthermore, the integration of molecular diagnostics into clinical practice has enhanced the accuracy of skin disorder diagnoses. Polymerase chain reaction (PCR), next-generation sequencing (NGS), and other molecular assays have allowed for the detection of infectious agents, assessment of genetic mutations, and profile gene expression patterns with unequalled precision. These techniques have proven instrumental in distinguishing between subtypes of skin cancers, aiding treatment strategies and prognostic assessments. Moreover, molecular profiling is increasingly guiding the selection of therapeutic agents, ensuring a personalised and effective approach to managing skin disorders. The application of PCR has revolutionised the field by enabling the identification of microbial DNA (i.e., Mycobacterium tuberculosis and Epstein-Barr Virus) in skin infections and detecting specific genetic mutations associated with dermatological disorders (e.g., BRAF). DNA sequencing technologies, such as next-generation sequencing, have facilitated the elucidation of genetic variations and mutations in skin diseases (i.e., bullous disorders), paving the way for personalised treatment approaches. Gene expression profiling techniques, such as microarrays and RNA sequencing, have provided insights into dysregulated pathways and molecular signatures associated with conditions ranging from inflammatory skin disorders to cutaneous malignancies. Immunohistochemistry and fluorescence in situ hybridization have proven invaluable in determining protein expression patterns and detecting chromosomal abnormalities, respectively, aiding in the characterization of skin lesions in conjunction with the molecular data. Proteomic studies have contributed to understanding the intricate protein networks involved in dermatological conditions (i.e., psoriasis), while epigenetic analyses have shed light on the role of epigenetic modifications in gene regulation within skin cancer (i.e., Malignant Melanoma). Together, these molecular techniques have laid the groundwork for targeted therapies and precision medicine in dermatology, with implications for improved diagnostics and treatment outcomes. This review focuses on the routinely employed molecular techniques within dermatopathology, with a focus on cutaneous malignancies, autoimmune diseases, infectious diseases, and neonatal screening which can be implemented in the diagnosis and contribute to improved patient care.

J. A. Gabriel

N. Weerasinghe

P. Balachandran

R. Salih

G. E. Orchard

British Journal of Biomedical Science

《英国生物医学科学杂志》致力于发表代表生物医学科学实践明显进步的高质量原创研究，以及总结生物医学科学领域最新进展的评论。该杂志的总体目标是为传播有关疾病诊断和管理的新信息和创新信息提供一个平台，这些信息对执业实验室科学家很有价值。

The British Journal of Biomedical Science is dedicated to publishing high-quality original research that represents significant advancements in the practice of biomedical science, as well as review articles summarizing the latest developments in the field. The overall aim of the journal is to provide a platform for disseminating new and innovative information regarding disease diagnosis and management, which is valuable to practicing laboratory scientists.

《British Journal of Biomedical Science》致力于发表高质量的原创研究，这些研究代表了生物医学科学实践的显著进步。同时，期刊也欢迎对生物医学科学领域最新进展的评论文章。该杂志的主要目标是为传播有关疾病诊断和管理的新信息和创新提供一个平台，这些信息对执业实验室科学家具有重要价值。

Category	Quartile
医学	4
医学, 医学实验技术	4

A Narrative Review of Molecular, Immunohistochemical and In-Situ Techniques in Dermatopathology

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Journal Info

Category	Quartile
MEDICAL LABORATORY TECHNOLOGY	2