In this study, sheep plasma was submitted to Alcalase-hydrolysis and peptides with better antioxidant properties measured through both the ferric-reducing antioxidant power (FRAP) and the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability assays were isolated and identified. After hydrolysate ultrafiltration and semi-preparative reverse-phase high-performance liquid chromatography, nine fractions (F1-F9) were obtained, with the two first (F1 and F2) showing the greatest antioxidant potential. These two fractions were further separated by the AKTA purifier system to generate four (F1-1-F1-4) and five (F2-1-F2-5) fractions, respectively, with two of them (F1-2 and F2-1) exhibiting appreciable FRAP activity and DPPH radical scavenging ability. Using liquid chromatography-tandem mass spectrometry, three antioxidant peptides were identified. From their amino acid sequences (QTALVELLK, SLHTLFGDELCK, and MPCTEDYLSLILNR), which include amino acids that have been previously reported as key contributors to the peptide antioxidant properties, it can be maintained that they come mainly from serum albumin. These results suggested that the sheep plasma protein can be considered as a good source of antioxidant peptides and bring forth new possibilities for the utilization of animal blood by-products.

Chengli Hou

Liguo Wu

Zhenyu Wang

Elena Saguer

Dequan Zhang

Antioxidants

抗氧化剂 (ISSN 2076-3921)，为与抗氧化剂科学和技术相关的研究提供了一个高级论坛。它发表研究论文、评论和通讯。我们的目标是鼓励科学家尽可能详细地发表他们的实验和理论结果。论文的长度没有限制。必须提供完整的实验细节，以便重现结果。有关计算或实验程序的全部细节的电子文件和软件，如果不能以正常方式发布，可以作为补充电子材料存放。

Antioxidants (ISSN 2076-3921) is a high-level forum dedicated to research related to the science and technology of antioxidants. The journal publishes research papers, reviews, and communications, aiming to encourage scientists to report their experimental and theoretical results in as much detail as possible. There is no limit on the length of papers, and complete experimental details must be provided to allow for the reproducibility of results. Any detailed electronic files and software related to computational or experimental procedures that cannot be published in the usual manner may be stored as supplementary electronic materials.

《Antioxidants》 (ISSN 2076-3921) 是一个专注于抗氧化剂科学与技术的高级论坛。期刊发表研究论文、评论和通讯，旨在鼓励科学家详细报告他们的实验和理论结果。论文长度没有限制，要求提供完整的实验细节，以便他人能够重现结果。如果计算或实验程序的详细信息无法以常规方式发布，可以将其作为补充电子材料存放。

Purification and Identification of Antioxidant Alcalase-Derived Peptides from Sheep Plasma Proteins

The results suggested that the sheep plasma protein can be considered as a good source of antioxidant peptides and bring forth new possibilities for the utilization of animal blood by-products.

Category	Quartile
医学	2
医学, 生化与分子生物学	2
医学, 药物化学	2
医学, 食品科技	2

Purification and Identification of Antioxidant Alcalase-Derived Peptides from Sheep Plasma Proteins

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Journal Info

Category	Quartile
FOOD SCIENCE & TECHNOLOGY	1