Nanofiber microspheres have attracted a lot of attention for biomedical applications because of their injectable and biomimetic properties. Herein, we report for the first time a new method for fabrication of nanofiber microspheres by combining electrospinning and electrospraying and explore their potential applications for cell therapy. Electrospraying of aqueous dispersions of electrospun nanofiber segments with desired length obtained by either cryocutting or homogenization into liquid nitrogen followed by freeze-drying and thermal treatment can form nanofiber microspheres. The microsphere size can be controlled by varying the applied voltage during the electrospray process. A variety of morphologies were achieved including solid, nanofiber, porous and nanofiber microspheres, and hollow nanofiber microspheres. Furthermore, a broad range of polymer and inorganic bioactive glass nanofiber-based nanofiber microspheres could be fabricated by electrospraying of their short nanofiber dispersions, indicating a comprehensive applicability of this method. A higher cell carrier efficiency of nanofiber microspheres as compared to solid microspheres was demonstrated with rat bone marrow-derived mesenchymal stem cells, along with the formation of microtissue-like structures in situ, when injected into microchannel devices. Also, mouse embryonic stem cells underwent neural differentiation on the nanofiber microspheres, indicated by positive staining of β-III-tubulin and neurite outgrowth. Taken together, we developed a new method for generating nanofiber microspheres that are injectable and have improved viability and maintenance of stem cells for potential application in cell therapy.

Sunil Kumar Boda

Shixuan Chen

Kathy Chu

Hyung Joon Kim

Jingwei Xie

ACS Applied Materials & Interfaces

ACS APPLIED MATERIALS & INTERFACES 是一本面向化学家、工程师、物理学家和生物学家的跨学科社区的新出版物，重点关注如何开发新发现的材料和界面过程并将其用于特定应用。该杂志除了发表致力于推进应用材料研究的评论和论坛外，还发表来自学术界和工业界研究人员的完整文章和信件。

ACS Applied Materials and Interfaces is an interdisciplinary journal aimed at chemists, engineers, physicists, and biologists, focusing on the development of newly discovered materials and interface processes for specific applications. The journal publishes not only reviews and forums that advance applied materials research but also full articles and letters from researchers in academia and industry.

《ACS Applied Materials and Interfaces》是一本面向化学家、工程师、物理学家和生物学家的跨学科期刊，专注于新材料和界面过程的开发及其特定应用。该杂志不仅发表推动应用材料研究的评论和论坛，还刊登来自学术界和工业界研究人员的完整文章和信件。

ACS Applied Materials and Interfaces

Electrospraying Electrospun Nanofiber Segments into Injectable Microspheres for Potential Cell Delivery

A new method for generating nanofiber microspheres that are injectable and have improved viability and maintenance of stem cells for potential application in cell therapy is developed.

Category	Quartile
材料科学	2
材料科学, 材料科学综合	2
材料科学, 纳米科技	2

Electrospraying Electrospun Nanofiber Segments into Injectable Microspheres for Potential Cell Delivery

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Journal Info

Category	Quartile
NANOSCIENCE & NANOTECHNOLOGY	1