Abstract The rapid spreading of the disease during last few years highlighted the need of a quick, sensitive and reliable method for Pseudomonas syringae pv. actinidiae (Psa) detection, to find possible inoculum sources and limit the pathogen spreading. A PCR method, using new primers designed on the gene encoding a putative outer membrane protein P1 , was developed to detect Psa in symptomatic and asymptomatic tissue; a nested‐ PCR was also applied. Bleeding sap samples, collected in early spring from orchards with symptomatic and asymptomatic trees, were used both for PCR assays and for pathogen isolation and identification. The PCR and nested PCR methods were able to detect Psa presence at very low concentration from plant and pollen extracts; RFLP analyses with Bcl I on PCR and nested PCR amplicons confirmed the assay specificity, while the digestion with Bfm I and Alu I allowed to discriminate Psa strains isolated before 2008 from those isolated after 2008. Furthermore, the PCR and nested PCR on crude bleeding sap samples detected the presence of the pathogen in 3 and 5 of the 15 assayed samples, respectively. Direct isolation from the same samples and bacterial identification confirmed the results of molecular analysis.

E. Biondi

A. Galeone

N. Kuzmanović

S. Ardizzi

C. Lucchese

A. Bertaccini

Annals of Applied Biology

ANNALS OF APPLIED BIOLOGY 是由应用生物学家协会赞助的国际期刊。该杂志发表关于作物生产、作物保护和种植生态系统应用研究各个方面的原创研究论文。该杂志每年以在线和六期印刷的形式出版。年刊论文必须对知识的进步做出重大贡献，并且可能包括以下科学学科：农学农业气象学农业环境科学应用基因组学应用代谢组学应用蛋白质组学生物多样性生物控制气候变化作物生态昆虫学遗传操作分子生物学真菌学线虫学害虫植物病理学植物育种和遗传学植物生理学收获后生物学土壤科学统计学病毒学杂草生物学年鉴也欢迎对这些学科领域感兴趣的评论。评论应该是对该领域的批判性调查，并提供新的见解。所有论文均需经过同行评审。论文通常必须对应用生物学知识的进步做出重大贡献，但讨论技术或证实结果的简短论文，以及对应用生物学家感兴趣的当前知识的评论将被考虑发表。论文或评论不得提供给任何其他期刊以供事先或同时发表，通常平均为七页。

The Annals of Applied Biology is an international journal sponsored by the Association of Applied Biologists, focusing on original research in the fields of crop production, crop protection, and the application of research in agro-ecosystems. The journal publishes six issues annually in both online and print formats. Submitted papers must make a significant contribution to the advancement of knowledge and may cover various scientific disciplines, including agronomy, agricultural meteorology, agricultural environmental science, applied genomics, applied metabolomics, applied proteomics, biodiversity, biocontrol, climate change, crop ecology, entomology, genetic engineering, molecular biology, mycology, nematology, pest management, plant pathology, plant breeding and genetics, plant physiology, post-harvest biology, soil science, statistics, virology, and weed biology. The journal also welcomes critical reviews in these fields that provide new insights. All submissions undergo peer review. Papers are generally expected to make a significant contribution to the advancement of applied biological knowledge, but brief papers discussing techniques or confirming results, as well as reviews of current knowledge of interest to applied biologists, will also be considered for publication. Submissions must not be offered to any other journal for prior or simultaneous publication, and papers typically average seven pages in length.

《Annals of Applied Biology》是由应用生物学家协会赞助的国际期刊，专注于作物生产、作物保护和种植生态系统的应用研究。该期刊每年在线和印刷版各出版六期，发表原创研究论文，要求对知识的进步做出显著贡献。涉及的科学领域包括：农学、农业气象学、农业环境科学、应用基因组学、应用代谢组学、应用蛋白质组学、生物多样性、生物控制、气候变化、作物生态、昆虫学、遗传操作、分子生物学、真菌学、线虫学、害虫学、植物病理学、植物育种与遗传学、植物生理学、收获后生物学、土壤科学、统计学、病毒学和杂草生物学。此外，期刊也欢迎对这些领域感兴趣的评论文章，要求对该领域进行批判性调查并提供新的见解。所有论文均需经过同行评审，通常要求对应用生物学知识的进步做出重大贡献。简短的技术讨论或结果验证论文，以及对应用生物学家感兴趣的当前知识的评论也会被考虑发表。所有提交的论文或评论不得同时或提前提交给其他期刊，通常平均长度为七页。

Ann Appl Biol

<i>Pseudomonas syringae</i>pv.<i>actinidiae</i>detection in kiwifruit plant tissue and bleeding sap

A PCR method, using new primers designed on the gene encoding a putative outer membrane protein P1, was developed to detect Psa in symptomatic and asymptomatic tissue and detected the presence of the pathogen in 3 and 5 of the 15 assayed samples.

Category	Quartile
农林科学	3
农林科学, 农业综合	3

Pseudomonas syringaepv.actinidiaedetection in kiwifruit plant tissue and bleeding sap

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Journal Info

Category	Quartile
AGRICULTURE, MULTIDISCIPLINARY	2