Respiratory syncytial virus (RSV) infection is the primary cause of respiratory disease in infants. The formalin-inactivated RSV (FI-RSV) vaccine resulted in an enhanced respiratory disease (ERD) in infants upon natural RSV infection, which is a major obstacle for development of safe and efficacious vaccines. Excessive and uncontrolled Th immune responses could be involved in the ERD. Agonists of TLRs are used as adjuvants to guide the type of immune response induced by vaccines. We evaluated the impact of lipopolysaccharide (LPS), the agonist of TLR4, on ERD as the adjuvant of FI-RSV. The results showed that LPS remarkably inhibited FI-RSV-enhanced lung inflammation, mucus production, airway inflammatory cell infiltration, and inflammatory cytokines following RSV challenge. Interestingly, LPS inhibited both Th2 and Th17 type cytokines in lungs of FI-RSV-immunized mice following RSV challenge, without an increase in the Th1 type cytokines, suggesting a controlled immune response. In contrast, Pam3Cys and Poly(I:C), the agonist of TLR1/2 or TLR3, partly inhibited FI-RSV-enhanced lung inflammation. Pam3Cys inhibited Th17 type cytokine IL-17, but promoted both Th1 and Th2 type cytokines. Poly(I:C) inhibited Th2 and Th17 type cytokines, but promoted Th1 type cytokines. In addition, LPS promoted IgG and IgG2a antibody production, which might provide protection from RSV challenge. These results suggest that LPS inhibits ERD without impairment in antibody production and protection, and the mechanism appears to be related with regulation of Th responses induced by FI-RSV.

Wei Yin

Hong-Yong Li

Bo-Yang Zheng

Yu-Qing Deng

Wen-Jian Li

Ying Liu

Rui-Hong Zeng

Current medical science

CURRENT MEDICAL SCIENCE 为医学科学领域的同行评议论文提供论坛，以促进中国研究人员和医生与外国同行之间的学术交流。期刊涵盖生理学、生物化学、分子生物学、药理学、病理学和病理生理学等生物医学学科和外科、内科、妇产科、儿科、耳鼻喉科等临床研究学科。 MEDICAL SCIENCE 以英文为主，极少数论文为德文，以向其德国创始人致敬。本刊是中国中部地区教育机构主办的唯一西文医学期刊。

Current Medical Science is a peer-reviewed journal that provides a forum for papers in the field of medical science, aimed at promoting academic exchange between Chinese researchers and physicians and their international counterparts. The journal covers biomedical disciplines such as physiology, biochemistry, molecular biology, pharmacology, pathology, and pathophysiology, as well as clinical research fields including surgery, internal medicine, obstetrics and gynecology, pediatrics, and otorhinolaryngology. The journal primarily publishes in English, with a very limited number of papers in German, in honor of its German founder. It is the only Western medical journal published by educational institutions in Central China.

《Current Medical Science》是一个为医学科学领域的同行评审论文提供论坛的期刊，旨在促进中国研究人员和医生与国际同行之间的学术交流。该期刊涵盖生理学、生物化学、分子生物学、药理学、病理学和病理生理学等生物医学学科，以及外科、内科、妇产科、儿科、耳鼻喉科等临床研究领域。期刊主要以英文发表，极少数论文为德文，以向其德国创始人致敬。本刊是中国中部地区教育机构主办的唯一西文医学期刊。

Current Medical Science

Curr Med Sci

Lipopolysaccharide Inhibits FI-RSV Vaccine-enhanced Inflammation Through Regulating Th Responses.

The results suggest that LPS inhibits ERD without impairment in antibody production and protection, and the mechanism appears to be related with regulation of Th responses induced by FI-RSV.

Category	Quartile
医学	4
医学, 医学研究与实验	4

Lipopolysaccharide Inhibits FI-RSV Vaccine-enhanced Inflammation Through Regulating Th Responses.

ivySCI AI Smartly Parses PDF, Answers Researchers' Questions, and Helps You Understand Papers in Seconds

Journal Info

Category	Quartile
MEDICINE, RESEARCH & EXPERIMENTAL	3