CRISPR/Cas systems confer immunity against invading nucleic acids and phages in bacteria and archaea. CRISPR/Cas13a (known previously as C2c2) is a class 2 type VI-A ribonuclease capable of targeting and cleaving single-stranded RNA (ssRNA) molecules of the phage genome. Here, we employ CRISPR/Cas13a to engineer interference with an RNA virus, Turnip Mosaic Virus (TuMV), in plants. CRISPR/Cas13a produces interference against green fluorescent protein (GFP)-expressing TuMV in transient assays and stable overexpression lines of Nicotiana benthamiana. CRISPR RNA (crRNAs) targeting the HC-Pro and GFP sequences exhibit better interference than those targeting other regions such as coat protein (CP) sequence. Cas13a can also process pre-crRNAs into functional crRNAs. Our data indicate that CRISPR/Cas13a can be used for engineering interference against RNA viruses, providing a potential novel mechanism for RNA-guided immunity against RNA viruses and for other RNA manipulations in plants.

Rashid Aman

Zahir Ali

Haroon Butt

Ahmed Mahas

Fatimah Aljedaani

Muhammad Zuhaib Khan

Shouwei Ding

Magdy Mahfouz

Genome biology

基因组生物学涵盖从基因组和后基因组角度研究的生物学和生物医学的所有领域。内容包括研究、新方法和软件工具，以及评论、意见和评论。涵盖的领域包括但不限于：序列分析；生物信息学；深入了解分子、细胞和有机体生物学；功能基因组学；表观基因组学；群体基因组学；蛋白质组学；比较生物学和进化论；系统和网络生物学；基因组编辑和工程；疾病基因组学；和临床基因组学。

Genome Biology covers all areas of biology and biomedicine studied from the perspectives of genomics and post-genomics. The content includes research articles, new methods and software tools, as well as reviews and opinions. The fields covered include, but are not limited to: sequence analysis; bioinformatics; in-depth studies of molecular, cellular, and organismal biology; functional genomics; epigenomics; population genomics; proteomics; comparative biology and evolution; systems and network biology; genome editing and engineering; disease genomics; and clinical genomics.

《Genome Biology》期刊涵盖了从基因组和后基因组的角度研究生物学和生物医学的各个领域。内容包括研究论文、新方法和软件工具，以及评论和意见。涉及的领域包括但不限于：序列分析、生物信息学、分子、细胞和有机体生物学的深入研究、功能基因组学、表观基因组学、群体基因组学、蛋白质组学、比较生物学和进化论、系统和网络生物学、基因组编辑和工程、疾病基因组学以及临床基因组学。

Genome Biology

Genome Biol

RNA virus interference via CRISPR/Cas13a system in plants

The data indicate that CRISPR/Cas13a can be used for engineering interference against RNA viruses, providing a potential novel mechanism for RNA-guided immunity againstRNA viruses and for other RNA manipulations in plants.

RNA virus interference via CRISPR/Cas13a system in plants

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Journal Info

Category	Quartile
GENETICS & HEREDITY	1