Polymerase chain reaction (PCR) has become a powerful tool for detecting various diseases due to its high sensitivity and specificity. However, the long thermocycling time and the bulky system have limited the application of PCR devices in Point-of-care testing. Herein, we have proposed an efficient, low-cost, and hand-hold PCR microdevice, mainly including a control module based on water-cooling technology and an amplification module fabricated by 3D printing. The whole device is tiny and can be easily hand-held with a size of about 110 mm × 100 mm × 40 mm and a weight of about 300 g at a low cost of about $170.83. Based on the water-cooling technology, the device can efficiently perform 30 thermal cycles within 46 min at a heating/cooling rate of 4.0/8.1 ℃/s. To test our instrument, plasmid DNA dilutions were amplified with this device; the results demonstrate successful nucleic acid amplification of the plasmid DNA and exhibit the promise of this device for Point-of-care testing.

Kaixin sun

Ben Whiteside

Michael Hebda

Yiqiang Fan

Yajun Zhang

Yumeng Xie

KunMing Liang

Biomedical Microdevices

BIOMEDICAL MICRODEVICES: BIOMEMS AND BIOMEDICAL NANOTECHNOLOGY 是一本跨学科期刊，致力于研究微机电系统 (BIOMEMS) 和纳米技术在医学和生物学中的医学诊断和治疗应用的各个方面。感兴趣的一般主题包括设计、表征、微制造系统的测试、建模和临床验证，以及它们在芯片上和更大功能单元中的集成。该杂志的具体兴趣包括神经刺激和记录系统、生物分离技术（如纳米过滤器和电泳设备）、小型化分析和 DNA 识别系统、生物传感器以及用于细胞和组织研究、组织工程、细胞移植和药物和生物分子的受控释放。鼓励对生物医学微型器件和纳米技术的材料科学、生物化学和物理学的基础和应用研究进行报告。感兴趣的领域的非详尽列表包括：纳米粒子合成、表征和动物模型中治疗或成像功效的验证；生物相容性；微制造装置的生化修饰，参考非特异性蛋白质吸附，以及微/纳米制造表面上蛋白质的主动固定和图案化；微纳通道中的流体动力学；微/纳米加工系统的机电和结构响应；微型设备与细胞和组织的相互作用，包括生物相容性和生物降解研究；系统特性的变化作为微/纳米加工参数的函数。

Biomedical Microdevices is an interdisciplinary journal dedicated to the study of microelectromechanical systems (BIOMEMS) and the applications of nanotechnology in medical diagnostics and therapeutics in the fields of medicine and biology. The journal covers a wide range of topics including design, characterization, testing of microfabrication systems, modeling, and clinical validation, as well as the integration of these technologies in chips and larger functional units. Specific areas of interest include neural stimulation and recording systems, bioseparation technologies (such as nanofilters and electrophoresis devices), miniaturized analytical and DNA recognition systems, biosensors, and controlled release of drugs and biomolecules for cell and tissue studies, tissue engineering, and cell transplantation. The journal encourages reports on fundamental and applied research in materials science, biochemistry, and physics related to biomedical microdevices and nanotechnology. Areas of interest include but are not limited to: synthesis and characterization of nanoparticles and validation of therapeutic or imaging efficacy in animal models; biocompatibility; biochemical modification of microfabricated devices, particularly regarding nonspecific protein adsorption, and active immobilization and patterning of proteins on micro/nanofabricated surfaces; fluid dynamics in micro/nanofluidic channels; electromechanical and structural responses of micro/nanofabrication systems; interactions of microdevices with cells and tissues, including studies on biocompatibility and biodegradability; and variations in system characteristics as a function of micro/nanofabrication parameters.

《Biomedical Microdevices》是一本跨学科的期刊，专注于微机电系统（BIOMEMS）和纳米技术在医学和生物学领域的诊断和治疗应用。期刊涵盖的主题包括设计、表征、微制造系统的测试、建模和临床验证，以及这些技术在芯片和更大功能单元中的集成。具体研究兴趣包括神经刺激和记录系统、生物分离技术（如纳米过滤器和电泳设备）、小型化分析和DNA识别系统、生物传感器，以及用于细胞和组织研究、组织工程、细胞移植和药物与生物分子的受控释放。期刊鼓励对生物医学微型器件和纳米技术的材料科学、生物化学和物理学的基础与应用研究进行报告。感兴趣的领域包括但不限于：纳米粒子的合成、表征及其在动物模型中的治疗或成像效果验证；生物相容性；微制造装置的生化修饰，特别是针对非特异性蛋白质吸附的研究，以及在微/纳米制造表面上蛋白质的主动固定和图案化；微纳通道中的流体动力学；微/纳米加工系统的机电和结构响应；微型设备与细胞和组织的相互作用，包括生物相容性和生物降解研究；以及系统特性如何随微/纳米加工参数的变化而变化。

Category	Quartile
医学	4
医学, 工程生物医学	4
医学, 纳米科技	4

A low-cost and hand-hold PCR microdevice based on water-cooling technology

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