As the second most abundant polysaccharide in nature, hemicellulose can be degraded to xylose as the feedstock for bioconversion to fuels and chemicals. To enhance xylose conversion, the engineered Saccharomyces cerevisiae with xylose metabolic pathway is usually adapted with xylose as the carbon source in the laboratory. However, the mechanism under the adaptation phenomena of the engineered strain is still unclear.In this study, xylose-utilizing S. cerevisiae was constructed and used for the adaptation study. It was found that xylose consumption rate increased 1.24-fold in the second incubation of the yYST12 strain in synthetic complete-xylose medium compared with the first incubation. The study figured out that it was observed at the single-cell level that the stagnation time for xylose utilization was reduced after adaptation with xylose medium in the microfluidic device. Such transient memory of xylose metabolism after adaptation with xylose medium, named "xylose consumption memory", was observed in the strains with both xylose isomerase pathway and xylose reductase and xylitol dehydrogenase pathways. In further, the proteomic acetylation of the strains before and after adaptation was investigated, and it was revealed that H4K5 was one of the most differential acetylation sites related to xylose consumption memory of engineered S. cerevisiae. We tested 8 genes encoding acetylase or deacetylase, and it was found that the knockout of the GCN5 and HPA2 encoding acetylases enhanced the xylose consumption memory.The behavior of xylose consumption memory in engineered S. cerevisiae can be successfully induced with xylose in the adaptation. H4K5Ac and two genes of GCN5 and HPA2 are related to xylose consumption memory of engineered S. cerevisiae during adaptation. This study provides valuable insights into the xylose adaptation of engineered S. cerevisiae.

Yong-Shui Tan

Li Wang

Ying-Ying Wang

Qi-En He

Zhi-Hua Liu

Zhen Zhu

Kai Song

Bing-Zhi Li

Ying-Jin Yuan

Biotechnology for Biofuels

BIOTECHNOLOGY FOR BIOFUELS 是一本开放获取的同行评议期刊，以描述生物燃料、化学品和其他生物产品生产中的技术和操作进步的高质量研究为特色。该杂志强调理解和推进生物技术和协同操作的应用，以改进植物和生物转化系统，以利用生物质、生物质衍生的中间体或二氧化碳进行生物生产这些产品，以及生物不可或缺的上游或下游操作生物质转化。生物燃料的生物技术专注于以下领域：• 陆地植物原料的开发• 藻类原料的开发• 用于生物转化的生物质预处理、分馏和提取• 酶工程、生产和分析• 细菌遗传学、生理学和代谢工程•真菌/酵母遗传学、生理学和代谢工程• 发酵、生物催化转化和反应动力学• 从生物质中生物生产化学品和生物产品• 厌氧消化、生物氢和生物电• 生物工艺集成、技术经济分析、建模和政策• L IFE循环评估和环境影响分析

Biotechnology for Biofuels is an open-access, peer-reviewed journal that features high-quality research describing technological and operational advancements in the production of biofuels, chemicals, and other bioproducts. The journal emphasizes understanding and advancing the applications of biotechnology and collaborative operations to improve plant and bioconversion systems for the biological production of these products using biomass, biomass-derived intermediates, or carbon dioxide, as well as the upstream and downstream operations essential for biomass conversion. The biotechnology of biofuels focuses on the following areas: • Development of terrestrial plant feedstocks • Development of algal feedstocks • Pretreatment, fractionation, and extraction of biomass for bioconversion • Enzyme engineering, production, and analysis • Bacterial genetics, physiology, and metabolic engineering • Fungal/yeast genetics, physiology, and metabolic engineering • Fermentation, biocatalytic conversion, and reaction kinetics • Bioproduction of chemicals and bioproducts from biomass • Anaerobic digestion, biohydrogen, and bioelectricity • Integration of bioprocesses, techno-economic analysis, modeling, and policy • Life cycle assessment and environmental impact analysis.

《Biotechnology for Biofuels》是一本开放获取的同行评审期刊，专注于生物燃料、化学品及其他生物产品生产中的技术与操作进展。该期刊强调理解和推动生物技术及协同操作的应用，以改进植物和生物转化系统，利用生物质、生物质衍生的中间体或二氧化碳进行生物生产。这些研究涵盖了生物质转化的上游和下游操作。生物燃料的生物技术主要集中在以下领域：• 陆地植物原料的开发 • 藻类原料的开发 • 生物质的预处理、分馏和提取 • 酶工程、生产与分析 • 细菌的遗传学、生理学和代谢工程 • 真菌/酵母的遗传学、生理学和代谢工程 • 发酵、生物催化转化和反应动力学 • 从生物质中生物生产化学品和生物产品 • 厌氧消化、生物氢和生物电 • 生物工艺集成、技术经济分析、建模和政策 • 生命周期评估和环境影响分析

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Protein acetylation regulates xylose metabolism during adaptation of Saccharomyces cerevisiae

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