MicroRNA164 (miR164) plays a key role in leaf and flower development, lateral root initiation, and stress responses. However, little is known about the regulatory roles of miR164 during seed development, particularly in maize. The aim of this study was to discover the developmental function of miR164 in maize seed. Small RNA sequencing (sRNA-seq) was performed at two key stages. The results indicated that miR164 was down-regulated during maize seed development. In addition, degradome library sequencing and transient expression assays identified the target genes for miR164. Two microRNA (miRNA) pairs, miR164-NAM, ATAF, and CUC32 (NAC32) and miR164-NAC40, were isolated. The developmental function of miR164 was determined by analyzing the differentially expressed genes (DEGs) between the wild-type and miR164 transgenic lines using RNA sequencing (RNA-seq) and by screening the DEGs related to NAC32 and NAC40 via co-expression and transient expression analysis. These results identified two beta-expansin genes, EXPB14 and EXPB15, which were located downstream of the NAC32 and NAC40 genes. This study revealed, for the first time, a miR164-dependent regulatory pathway, miR164-NAC32/NAC40-EXPB14/EXPB15, which participates in maize seed expansion. These findings highlight the significance of miR164 in maize seed development, and can be used to explore the role of miRNA in seed development.

Lanjie Zheng

Xiangge Zhang

Haojun Zhang

Yong Gu

Xinrong Huang

Huanhuan Huang

Hanmei Liu

Junjie Zhang

Yufeng Hu

Yangping Li

Guowu Yu

Yinghong Liu

Shaneka S Lawson

Yubi Huang

Molecular genetics and genomics : MGG

分子遗传学和基因组学 (MGG) 发表同行评议的文章，涵盖遗传学和基因组学的所有领域。任何研究基因和基因组的方法都被考虑在内，无论是实验的、理论的还是合成的。 MGG 发表对遗传学、基因组学、生物学、医学和生物技术领域工作人员广泛感兴趣的所有生物的研究。该杂志调查了广泛的主题，包括最近几期的主题：延长寿命的机制生物体；筛选涉及线粒体功能的酵母金属稳态基因；稻瘟病菌品种特异性无毒基因的分子作图等。

Molecular Genetics and Genomics (MGG) publishes peer-reviewed articles covering all areas of genetics and genomics. We welcome any research methods related to genes and genomes, whether experimental, theoretical, or synthetic. The research published in this journal is of broad interest to professionals in genetics, genomics, biology, medicine, and biotechnology. The topics we explore are diverse, including recent themes such as mechanisms of lifespan extension; screening of yeast metal homeostasis genes involved in mitochondrial function; and molecular mapping of variety-specific avirulence genes in rice blast fungus.

《Molecular Genetics and Genomics》是一本发表同行评审文章的期刊，涵盖遗传学和基因组学的各个领域。我们欢迎任何研究基因和基因组的方法，无论是实验性的、理论性的还是合成性的。该期刊发表的研究对遗传学、基因组学、生物学、医学和生物技术领域的专业人士具有广泛的吸引力。我们探讨的主题范围广泛，包括最近几期的研究：延长寿命的机制；筛选与线粒体功能相关的酵母金属稳态基因；以及稻瘟病菌品种特异性无毒基因的分子作图等。

Molecular Genetics and Genomics

Mol Genet Genomics

The miR164-dependent regulatory pathway in developing maize seed.

A miR164-dependent regulatory pathway is revealed, for the first time, which participates in maize seed expansion and can be used to explore the role of miRNA in seed development.

Category	Quartile
生物学	3
生物学, 生化与分子生物学	3
生物学, 遗传学	3

The miR164-dependent regulatory pathway in developing maize seed.

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Journal Info

Category	Quartile
GENETICS & HEREDITY	3