Integral membrane proteins (IMPs) play central roles in cellular physiology and represent the majority of known drug targets. Single-molecule fluorescence and fluorescence resonance energy transfer (FRET) methods have recently emerged as valuable tools for investigating structure-function relationships in IMPs. This review focuses on the practical foundations required for examining polytopic IMP function using single-molecule FRET (smFRET) and provides an overview of the technical and conceptual frameworks emerging from this area of investigation. In this context, we highlight the utility of smFRET methods to reveal transient conformational states critical to IMP function and the use of smFRET data to guide structural and drug mechanism-of-action investigations. We also identify frontiers where progress is likely to be paramount to advancing the field.

Arnab Modak

Zeliha Kilic

Kanokporn Chattrakun

Daniel S. Terry

Ravi C. Kalathur

Scott C. Blanchard

Annual Review of Biophysics

自 1972 年出版的《生物物理学年度回顾》涵盖了生物物理学领域的重大发展，包括大分子结构、功能和动力学、理论和计算生物物理学、细胞的分子生物物理学、物理系统生物学、膜生物物理学、生物技术、纳米技术、和新兴技术。

Published since 1972, the Annual Review of Biophysics covers significant developments in the field of biophysics, including macromolecular structure, function and dynamics, theoretical and computational biophysics, molecular biophysics of cells, physical systems biology, membrane biophysics, biotechnology, nanotechnology, and emerging technologies.

《Annual Review of Biophysics》自1972年出版，涵盖了生物物理学领域的重大进展，包括大分子结构、功能和动力学、理论与计算生物物理学、细胞分子生物物理学、物理系统生物学、膜生物物理学、生物技术、纳米技术以及新兴技术。

Category	Quartile
生物学	1
生物学, 生物物理	1

Single-Molecule Imaging of Integral Membrane Protein Dynamics and Function

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