Abstract Epigenetic analysis is a fundamental part of understanding pathophysiological processes with potential applications in diagnosis, prognosis, and assessment of disease susceptibility. Epigenetic changes have been widely studied in chronic obstructive pulmonary disease (COPD), but currently, there is no molecular marker used to improve the treatment of patients. Furthermore, this progressive disease is a risk factor for the development of more severe COVID-19. Methylation-specific polymerase chain reaction (MSP-PCR) plays an important role in the analysis of DNA methylation profiles, and it is one of the most widely used techniques. In this context, the combination of MSP-PCR with emerging PCR technologies, such as digital PCR (dPCR), results in more accurate analyses of the DNA methylation profile of the genes under study. In this study, we propose the application of the MSP-dPCR technique to evaluate the methylation profile of the ADAM33 gene from saliva samples and lung tissue biopsies of patients with COPD and COVID-19. MSP-dPCR generated a measurable prediction of gene methylation rate, with the potential application of this combined technology for diagnostic and prognostic purposes. It has also proven to be a powerful tool for liquid biopsy applications.

Gabriela Casani Cardoso

Fernando Augusto de Oliveira Ganzella

Guilherme Miniskiskosky

Regiane Stafim da Cunha

Edneia Amancio de Souza Ramos

BioMolecular Concepts

BioMolecular Concepts is a peer-reviewed open access journal fostering the integration of different fields of biomolecular research. The journal aims to provide expert summaries from prominent researchers, and conclusive extensions of research data leading to new and original, testable hypotheses. Aspects of research that can promote related fields, and lead to novel insight into biological mechanisms or potential medical applications are of special interest. Original research articles reporting new data of broad significance are also welcome.    Topics:  -cellular and molecular biology-  genetics and epigenetics-  biochemistry-  structural biology-  neurosciences-  developmental biology-  molecular medicine-  pharmacology-  microbiology-  plant biology and biotechnology.

Biomolecular Concepts is a peer-reviewed open access journal that fosters the integration of various fields of biomolecular research. The journal aims to provide expert summaries from prominent researchers and conclusive extensions of research data that lead to new and original, testable hypotheses. It particularly focuses on research aspects that can promote related fields and offer novel insights into biological mechanisms or potential medical applications. Original research articles reporting new data of broad significance are also welcome. Topics include: - Cellular and Molecular Biology - Genetics and Epigenetics - Biochemistry - Structural Biology - Neurosciences - Developmental Biology - Molecular Medicine - Pharmacology - Microbiology - Plant Biology and Biotechnology.

《Biomolecular Concepts》是一本经过同行评审的开放获取期刊，旨在促进不同生物分子研究领域的整合。该期刊致力于提供来自杰出研究者的专业总结，以及研究数据的结论性扩展，进而形成新的、可验证的假设。特别关注能够促进相关领域研究并为生物机制或潜在医学应用提供新见解的研究方面。同时，欢迎报告具有广泛意义的新数据的原创研究文章。  主题包括：- 细胞与分子生物学 - 遗传学与表观遗传学 - 生物化学 - 结构生物学 - 神经科学 - 发展生物学 - 分子医学 - 药理学 - 微生物学 - 植物生物学与生物技术。

Digital methylation-specific PCR: New applications for liquid biopsy

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