Shigella species, which are closely related to Escherichia coli, can easily be maintained and stored in the laboratory. This article includes protocols for preparation of routine growth conditions and media, for storage of the bacteria, and for monitoring of the presence of the virulence plasmid. © 2019 by John Wiley & Sons, Inc. Basic Protocol 1: Growth of S. flexneri from frozen stocks or agar stabs Basic Protocol 2: Growth of S. flexneri in rich liquid medium Alternate Protocol 1: Growth of S. flexneri in rich defined medium Alternate Protocol 2: Growth of S. flexneri in minimal medium Basic Protocol 3: Storage of S. flexneri in frozen stocks Alternate Protocol 3: Storage of S. flexneri in agar stabs.

Shelley M. Payne

Current Protocols in Microbiology

Current Protocols in Microbiology provides detailed, step-by-step instructions for analyzing bacteria, animal and plant viruses, fungi, protozoans and other microbes. It offers updated coverage of emerging technologies and concepts, such as biofilms, quorum sensing and quantitative PCR, as well as proteomic and genomic methods. It is the first comprehensive source of high-quality microbiology protocols that reflects and incorporates the new mandates and capabilities of this robust and rapidly evolving discipline.

Current Protocols in Microbiology offers detailed, step-by-step instructions for analyzing bacteria, animal and plant viruses, fungi, protozoans, and other microbes. It provides updated coverage of emerging technologies and concepts, such as biofilms, quorum sensing, and quantitative PCR, as well as proteomic and genomic methods. It is the first comprehensive source of high-quality microbiology protocols that reflects and incorporates the new mandates and capabilities of this robust and rapidly evolving discipline.

《Current Protocols in Microbiology》提供了详细的逐步指导，帮助研究人员分析细菌、动物和植物病毒、真菌、原生动物及其他微生物。期刊涵盖了新兴技术和概念的最新进展，如生物膜、群体感应和定量PCR，以及蛋白质组学和基因组学方法。它是首个全面的高质量微生物学协议来源，反映并融入了这一快速发展的学科的新要求和能力。

Laboratory Cultivation and Storage of Shigella

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