This study aimed to improve the ability to detect the effects of radiation-associated genetic damage and establish a rapid and simple method for estimating biological dose.Radiation-sensitive genes were selected as biodosimeters from Pig-a,Gadd45α,and Hprt,based on their mRNA expression level changes.RT-qPCR was used to assay the relative expression level of the target genes in peripheral blood samples of healthy non-radiation(controlgroup)and radiation workers(exposed group)and their relative expression level average,fluctuation range and variation coefficient of the background expression were counted.Peripheral blood samples of healthy adults were irradiated with different X-ray doses of 0 Gy,0.10 Gy,0.25 Gy,0.50 Gy,1.00 Gy,2.00 Gy,and 5.00 Gy,respectively.6 h later,the relative expression of the target genes was examined using RT-qPCR and the binomial regression curve analysis was performed to analyze the quantitative effect relationship.The results showed that Piga and Gadd45αexpressions had small background level differences in the control group;Hprt had large background level differences and its variation coefficients were greater than 20%.The Hprt expression level in the control group was higher than that in the exposed group;Gadd45αand Pig-a expressions in the control group were lower than that in the exposed group(p<0.05).There was a dose-effect relationship between the Gadd45αand Pig-a expression levels and irradiation doses,indicating that the Pig-a and Gadd45αexpression level changes could be used as potential biodosimeters.

杨学琴

毛鑫玉

陈钰婷

李悦兰

Investigating the use of radiation-sensitive genes Pig-a,Gadd45α,and Hprt as potential biodosimeters

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