<h2>Abstract</h2> Human induced pluripotent stem (iPS) cell-derived intestinal organoids have low invasiveness; however, the current differentiation method does not reflect the crypt-villus-like structure due to structural immaturity. Here, we generated budding-like organoids that formed epithelial tissue-like structures and had the characteristics of the mature small intestine from human iPS cells. They showed a high expression of drug transporters and induced the expression of cytochrome P450 3A4 and P-glycoprotein. When treated with tumor necrosis factor-α and/or transforming growth factor-β, the budding-like organoids replicated the pathogenesis of mucosal damage or intestinal fibrosis. Upon dissociation and seeding on cell culture inserts, the organoids retained intestinal characteristics, forming polarized intestinal folds with approximately 400 Ω × cm<sup>2</sup> transepithelial electrical resistance. This novel method has great potential for disease modeling and drug screening applications.

Daichi Onozato

Isamu Ogawa

Yuriko Kida

Shota Mizuno

Tadahiro Hashita

Takahiro Iwao

Tamihide Matsunaga

Journal of Pharmaceutical Sciences

JOURNAL OF PHARMACEUTICAL SCIENCES 将发表原创研究论文、原创研究笔记、特邀专题评论（包括 MINIREVIEWS）以及社论评论和新闻。重点领域应为基础药物科学概念和药物化学加工等主题，包括结晶、冻干、药物化学稳定性、药代动力学、生物药剂学、药效学、前药开发、生物活性剂的代谢处置、剂型设计、蛋白质-肽化学和生物技术，特别是因为这些与制药技术和靶向药物输送有关。

The Journal of Pharmaceutical Sciences publishes original research articles, original research notes, invited topical reviews (including mini-reviews), as well as editorial comments and news. The focus areas should include fundamental concepts of pharmaceutical sciences and drug chemistry processing, covering topics such as crystallization, lyophilization, drug chemical stability, pharmacokinetics, biopharmaceutics, pharmacodynamics, prodrug development, metabolic disposition of bioactive agents, formulation design, protein-peptide chemistry, and biotechnology, particularly as they relate to pharmaceutical technology and targeted drug delivery.

《Journal of Pharmaceutical Sciences》将发表原创研究论文、研究笔记、特邀专题评论（包括小型综述）以及社论评论和新闻。重点领域包括基础药物科学概念和药物化学加工等主题，涵盖结晶、冻干、药物化学稳定性、药代动力学、生物药剂学、药效学、前药开发、生物活性剂的代谢处置、剂型设计、蛋白质-肽化学和生物技术，特别是与制药技术和靶向药物输送相关的研究。

Generation of Budding-Like Intestinal Organoids from Human Induced Pluripotent Stem Cells

B budding-like organoids were generated that formed epithelial tissue-like structures and had the characteristics of the mature small intestine from human iPS cells and showed a high expression of drug transporters and induced the expression of cytochrome P450 3A4 and P-glycoprotein.

Category	Quartile
医学	3
医学, 药物化学	3
医学, 化学综合	3
医学, 药学	3

Generation of Budding-Like Intestinal Organoids from Human Induced Pluripotent Stem Cells

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Journal Info

Category	Quartile
PHARMACOLOGY & PHARMACY	2