Conjugation of plasmids from Escherichia coli is essential for the genetic manipulation of Streptomyces spp. To facilitate intergeneric conjugation from E. coli to Streptomyces the conjugative machinery required for genetic transfer is usually provided by the non-transferable helper plasmid, pUZ8002. Here we present the complete nucleotide sequence of pUZ8002, describe the previously undocumented creation process, and provide details of the sequence relative to the parental pUZ8 plasmid and another previously published pUZ8002 sequence.

Daniel E. Larcombe

Robyn E. Braes

James T. Croxford

James W. Wilson

David H. Figurski

Paul A. Hoskisson

Access Microbiology

Sequence and origin of the Streptomyces intergenetic-conjugation helper plasmid pUZ8002

The complete nucleotide sequence of pUZ8002 is presented, the previously undocumented creation process is described, and details of the sequence relative to the parental pUZ8 plasmid and another previously published pUZ8002 sequence are provided.

Sequence and origin of the Streptomyces intergenetic-conjugation helper plasmid pUZ8002

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