Encapsulating cells within biocompatible materials is a widely pursued and promising element of tissue engineering and cell-based therapies. Recently, extensive interest in microfluidic-enabled cell encapsulation has emerged as a strategy to structure hydrogels and establish custom cellular microenvironments. In particular, it has been shown that the microfluidic-enabled photoencapsulation of cells within PEG diacrylate (PEGDA)-based microparticles can be performed cytocompatibly within gas-permeable, nitrogen-jacketed polydimethylsiloxane microfluidic devices, which mitigate the oxygen inhibition of radical chain growth photopolymerization. Compared to bulk polymerization, in which cells are suspended in a static hydrogel-forming solution during gelation, encapsulating cells via microfluidic processing exposes cells to a host of potentially deleterious stresses such as fluidic shear rate, transient oxygen depletion, elevated pressures, and UV exposure. In this work, we systematically examine the effects of these factors on the viability of cells that have been microfluidically photoencapsulated in PEGDA. It was found that the fluidic shear rate during microdroplet formation did not have a direct effect on cell viability, but the flow rate ratio of oil to aqueous solution would impart harmful effects to cells when a critical threshold was exceeded. The effects of UV exposure time and intensity on cells, however, are more complex, as they contribute unequally to the cumulative rate of peroxy radical generation, which is strongly correlated with cell viability. A reaction-diffusion model has been developed to calculate the cumulative peroxy radical concentration over a range of UV light intensity and radiation times, which was used to gain further quantitative understanding of experimental results. Conclusions drawn from this work provide a comprehensive guide to mitigate the physical and biochemical damage imparted to cells during microfluidic photoencapsulation and expands the potential for this technique.

Bingzhao Xia

Zhongliang Jiang

Daniel Debroy

Dongmei Li

John Oakey

Biomicrofluidics

BIOMICROFLUIDICS (BMF) 是 AIP PUBLISHING 出版的仅限在线期刊，旨在快速传播与微流体和纳米流体现象相关的基本物理化学机制的研究。 BMF 还发表用于诊断、医学、生物、制药、环境和化学应用的独特微流体和纳米流体技术的研究。BMF 提供快速出版、多媒体功能以及在学术、国家和工业实验室之间的全球流通。 BMF 主要关注高质量的原创研究文章，还组织了专门的章节，帮助解释和定义生物微流体跨学科领域特有的特定挑战。微流体和纳米流体驱动（电动、声流体、光流体、毛细管）液体活检（MICRORNA 分析、循环肿瘤细胞分离、外泌体分离、循环肿瘤DNA定量）细胞分选、操作和转染（DI/电泳、磁珠、光阱、电穿孔）分子分离和浓缩（等速电泳、浓差极化、DI/电泳、磁珠, 纳米颗粒）细胞培养和分析（单细胞测定、刺激反应、干细胞转染）基因组和蛋白质组分析（快速基因测序、DNA/蛋白质/碳水化合物阵列）生物传感器（免疫测定、核酸荧光测定、比色测定、酶放大，等离子体和拉曼纳米报告分子，分子 B EACON、FRET、适配体、纳米孔、光纤）生物物理传输和表征（DNA、单蛋白、离子通道和膜动力学、细胞运动和通信机制、电生理学、膜片钳）。 ETC...

Biomicrofluidics is an online journal published by AIP PUBLISHING, aimed at the rapid dissemination of research related to the fundamental physicochemical mechanisms of microfluidic and nanofluidic phenomena. The journal also publishes unique microfluidic and nanofluidic technologies for applications in diagnostics, medicine, biology, pharmaceuticals, environmental science, and chemistry. It offers rapid publication, multimedia features, and global circulation among academic, national, and industrial laboratories. Biomicrofluidics primarily focuses on high-quality original research articles and organizes dedicated sections to help explain and define the specific challenges unique to the interdisciplinary field of biomicrofluidics. Research topics include microfluidic and nanofluidic-driven liquid biopsies (such as MICRORNA analysis, circulating tumor cell isolation, exosome separation, and circulating tumor DNA quantification), cell sorting, manipulation, and transfection (such as DI/electrophoresis, magnetic beads, optical traps, and electroporation), molecular separation and concentration (such as isoelectric focusing, concentration polarization, DI/electrophoresis, magnetic beads, and nanoparticles), cell culture and analysis (such as single-cell assays, stimulation responses, and stem cell transfection), genomics and proteomics (such as rapid gene sequencing, DNA/protein/carbohydrate arrays), biosensors (such as immunoassays, nucleic acid fluorescence assays, colorimetric assays, enzyme amplification, plasmonic and Raman nano-reporters, molecular B EACON, FRET, aptamers, nanopores, and optical fibers), and biophysical transport and characterization (such as DNA, single proteins, ion channels and membrane dynamics, cell motility and communication mechanisms, electrophysiology, and patch clamping).

《Biomicrofluidics》是AIP PUBLISHING出版的在线期刊，旨在快速传播与微流体和纳米流体现象相关的基本物理化学机制的研究。该期刊还发表用于诊断、医学、生物、制药、环境和化学应用的独特微流体和纳米流体技术的研究。期刊提供快速出版、多媒体功能，并促进学术界、国家和工业实验室之间的全球交流。《Biomicrofluidics》主要关注高质量的原创研究文章，并组织专门章节，以帮助解释和定义生物微流体领域特有的挑战。研究主题包括微流体和纳米流体驱动的液体活检（如MICRORNA分析、循环肿瘤细胞分离、外泌体分离、循环肿瘤DNA定量）、细胞分选、操作和转染（如DI/电泳、磁珠、光阱、电穿孔）、分子分离和浓缩（如等速电泳、浓差极化、DI/电泳、磁珠、纳米颗粒）、细胞培养和分析（如单细胞测定、刺激反应、干细胞转染）、基因组和蛋白质组分析（如快速基因测序、DNA/蛋白质/碳水化合物阵列）、生物传感器（如免疫测定、核酸荧光测定、比色测定、酶放大、等离子体和拉曼纳米报告分子、分子B EACON、FRET、适配体、纳米孔、光纤）以及生物物理传输和表征（如DNA、单蛋白、离子通道和膜动力学、细胞运动和通信机制、电生理学、膜片钳等）。

Cytocompatible cell encapsulation via hydrogel photopolymerization in microfluidic emulsion droplets

Conclusions drawn from this work provide a comprehensive guide to mitigate the physical and biochemical damage imparted to cells during microfluidic photoencapsulation and expands the potential for this technique.

Category	Quartile
工程技术	4
工程技术, 生化研究方法	4
工程技术, 生物物理	4
工程技术, 纳米科技	4
工程技术, 物理流体与等离子体	4

Cytocompatible cell encapsulation via hydrogel photopolymerization in microfluidic emulsion droplets

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Journal Info

Category	Quartile
PHYSICS, FLUIDS & PLASMAS	2