Abstract Many bacteria can become naturally competent to take up extracellular DNA across their outer and inner membranes by a dedicated competence apparatus. Whereas some studies show that the DNA delivered to the cytoplasm may be used for genome repair or for nutrition, it can also be recombined onto the chromosome by homologous recombination: a process called natural transformation. Along with conjugation and transduction, natural transformation represents a mechanism for horizontal transfer of genetic material, e.g., antibiotic resistance genes, which can confer new beneficial characteristics onto the recipient bacteria. Described here are protocols for quantifying the frequency of transformation for the human pathogen Vibrio cholerae , one of several Vibrio species recently shown to be capable of natural transformation. © 2014 by John Wiley &amp; Sons, Inc.

Samit S. Watve

Eryn E. Bernardy

Brian K. Hammer

Current Protocols in Microbiology

Current Protocols in Microbiology provides detailed, step-by-step instructions for analyzing bacteria, animal and plant viruses, fungi, protozoans and other microbes. It offers updated coverage of emerging technologies and concepts, such as biofilms, quorum sensing and quantitative PCR, as well as proteomic and genomic methods. It is the first comprehensive source of high-quality microbiology protocols that reflects and incorporates the new mandates and capabilities of this robust and rapidly evolving discipline.

Current Protocols in Microbiology offers detailed, step-by-step instructions for analyzing bacteria, animal and plant viruses, fungi, protozoans, and other microbes. It provides updated coverage of emerging technologies and concepts, such as biofilms, quorum sensing, and quantitative PCR, as well as proteomic and genomic methods. It is the first comprehensive source of high-quality microbiology protocols that reflects and incorporates the new mandates and capabilities of this robust and rapidly evolving discipline.

《Current Protocols in Microbiology》提供了详细的逐步指导，帮助研究人员分析细菌、动物和植物病毒、真菌、原生动物及其他微生物。期刊涵盖了新兴技术和概念的最新进展，如生物膜、群体感应和定量PCR，以及蛋白质组学和基因组学方法。它是首个全面的高质量微生物学协议来源，反映并融入了这一快速发展的学科的新要求和能力。

<i>Vibrio cholerae</i>: Measuring Natural Transformation Frequency

Protocols for quantifying the frequency of transformation for the human pathogen Vibrio cholerae, one of severalVibrio species recently shown to be capable of natural transformation, are described.

Vibrio cholerae: Measuring Natural Transformation Frequency

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