ABSTRACT Deuterium‐labeled pyocyanin was prepared from deuterium‐labeled phenazine methosulfate in gram scale by a simplified flow photosynthesis in water. The main product was the protonated red form of pyocyanin‐d 3 (Pyo‐d 3 ‐H + ) in 85 % yield. Quantum chemical calculations of NMR support that nitrogen‐10 is protonated. The by‐products of the photolysis and the stability of the photolysis mixture were carefully characterized by LC‐MS and NMR. Four by‐products were identified: An isomer of pyocyanin‐d 3 (9%), 8‐hydroxypyocyanin‐d 3 (4%), 1‐hydroxyphenazine (0.4%), and phenazine (1%). The Pyo‐d 3 ‐H + product was stable in the photolysis solution after storage at 8°C for 2.5 years. Pure blue pyocyanin‐d 3 powder was isolated from the red photolysis solution by the Surrey method in 94 % yield. The addition of the red photolysis solution of Pyo‐d 3 ‐H + (100 μM) and commercial pyocyanin (100 μM) to Pseudomonas aeruginosa cultures showed the same growth curves demonstrating that the minor impurities in the photolysis solution do not affect the growth behavior of the bacteria. The protonated deuterium‐labeled pyocyanin may be used directly in biological experiments, which make the methodology extremely simple and useful for biologists.

Torben Lund

Niels Jacob Krake

Poul Erik Hansen

Fatima AlZahraa Alatraktchi

Journal of Labelled Compounds and Radiopharmaceuticals

《标记化合物和放射性药物杂志》发表了涉及标记化合物制备和这些化合物应用的研究的所有方面。这包括用于体外和体内医学、药理学、生物学、生化和化学研究的示踪方法。《标记化合物和放射性药物杂志》特别关注放射性药物的生物医学研究、诊断和治疗应用，涵盖从基础到发展的所有阶段基于物理和化学特征的分子结构、配位化合物和纳米粒子的代谢研究和技术开发到临床前和临床研究。

The Journal of Labelled Compounds and Radiopharmaceuticals publishes research covering all aspects of the preparation and application of labelled compounds. This includes tracing methods used in in vitro and in vivo medicine, pharmacology, biology, biochemistry, and chemical research. The journal places special emphasis on biomedical research involving radiopharmaceuticals, as well as their diagnostic and therapeutic applications, covering all stages from fundamental studies to preclinical and clinical research, focusing on the metabolic studies and technological developments of molecular structures, coordination compounds, and nanoparticles based on their physical and chemical characteristics.

《Journal of Labelled Compounds and Radiopharmaceuticals》发表涉及标记化合物制备及其应用的研究，涵盖所有相关领域。这些领域包括体外和体内医学、药理学、生物学、生化和化学研究中的示踪方法。该期刊特别关注放射性药物的生物医学研究及其在诊断和治疗中的应用，涵盖从基础研究到临床前和临床研究的各个阶段，涉及基于物理和化学特征的分子结构、配位化合物和纳米粒子的代谢研究与技术开发。

Category	Quartile
医学	4
医学, 生化研究方法	4
医学, 分析化学	4
医学, 药物化学	4

Simplified Flow Photosynthesis of Deuterium‐Labeled Pyocyanin

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Journal Info

Category	Quartile
CHEMISTRY, MEDICINAL	4