In order to unravel the genetic architecture underlying plant response to drought, we adopted an integrated approach, combining transcript profiling and quantitative trait loci (QTL) mapping. In fact, improving plant tolerance to water stress is an important, but, at the same time, a difficult task, since plant tolerance is the result of many complex mechanisms acting at different levels of plant organization, and its genetic basis is largely unknown. The phenotypic data, concerning yield components and flowering time, of a population of 142 maize Recombinant Inbred Lines (RILs), grown under well watered conditions or under water stress, were submitted to linkage analysis to detect drought-tolerance QTLs. Thirty genomic regions containing 50 significant QTLs distributed on nine chromosomes were identified. At the same time, a customized targeted oligoarray was used to monitor the expression levels of 1,000 genes, representative of the immature maize kernel transcriptome. Using this DNA array we compared transcripts from 10 days after pollination kernels of two susceptible and two drought tolerant genotypes (extracted from our RILs) grown under control and water stress field conditions. Two hundred and fifty-two genes were significantly affected by stress in at least one genotype. From a set of these, 49 new molecular markers were developed. By mapping most of them and by in silico mapping other regulated sequences, 88 differentially expressed genes were localized onto our linkage map, which, added to the existing 186 markers, brought their total number on the map to 274. Twenty-two of the 88 differentially expressed genes mapped in the same chromosomal segments harbouring QTLs for tolerance, thus representing candidate genes for further functional studies.

Rosanna Marino

Maharajah Ponnaiah

Pawel Krajewski

Carla Frova

Luca Gianfranceschi

M Enrico Pè

Mirella Sari-Gorla

Molecular genetics and genomics : MGG

分子遗传学和基因组学 (MGG) 发表同行评议的文章，涵盖遗传学和基因组学的所有领域。任何研究基因和基因组的方法都被考虑在内，无论是实验的、理论的还是合成的。 MGG 发表对遗传学、基因组学、生物学、医学和生物技术领域工作人员广泛感兴趣的所有生物的研究。该杂志调查了广泛的主题，包括最近几期的主题：延长寿命的机制生物体；筛选涉及线粒体功能的酵母金属稳态基因；稻瘟病菌品种特异性无毒基因的分子作图等。

Molecular Genetics and Genomics (MGG) publishes peer-reviewed articles covering all areas of genetics and genomics. We welcome any research methods related to genes and genomes, whether experimental, theoretical, or synthetic. The research published in this journal is of broad interest to professionals in genetics, genomics, biology, medicine, and biotechnology. The topics we explore are diverse, including recent themes such as mechanisms of lifespan extension; screening of yeast metal homeostasis genes involved in mitochondrial function; and molecular mapping of variety-specific avirulence genes in rice blast fungus.

《Molecular Genetics and Genomics》是一本发表同行评审文章的期刊，涵盖遗传学和基因组学的各个领域。我们欢迎任何研究基因和基因组的方法，无论是实验性的、理论性的还是合成性的。该期刊发表的研究对遗传学、基因组学、生物学、医学和生物技术领域的专业人士具有广泛的吸引力。我们探讨的主题范围广泛，包括最近几期的研究：延长寿命的机制；筛选与线粒体功能相关的酵母金属稳态基因；以及稻瘟病菌品种特异性无毒基因的分子作图等。

Molecular Genetics and Genomics

Mol Genet Genomics

Addressing drought tolerance in maize by transcriptional profiling and mapping.

In order to unravel the genetic architecture underlying plant response to drought, an integrated approach is adopted, combining transcript profiling and quantitative trait loci (QTL) mapping, which resulted in 88 differentially expressed genes being localized onto the linkage map.

Category	Quartile
生物学	3
生物学, 生化与分子生物学	3
生物学, 遗传学	3

Addressing drought tolerance in maize by transcriptional profiling and mapping.

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Journal Info

Category	Quartile
GENETICS & HEREDITY	3