A repeatable procedure for fertilization of bovine ova in vitro is described. Oocytes were recovered from ovarian follicles or from oviducts near the time of ovulation following treatment of donors with pregnant mare's serum gonadotropin (PMSG) and prostaglandin F2 alpha (PGF2 alpha). For in vitro capacitation semen was incubated, then high ionic strength treated and subsequently incubated in defined medium prior to insemination of oocytes. In one experiment frozen bull semen was successfully used. In experiments with 4 bulls (B, C, D, F), 34 (43.6%) of 78 ova and 13 (19.7%) of 66 follicular oocytes were fertilized in vitro. In the last series (spermatozoa from Bull F) the fertilization of 22 (62.9%) of 35 tubal ova was achieved. In vitro development proceeded to the 8-cell stage. No fertilization in vitro followed use of one male (Bull E), even though his spermatozoa could penetrate zona-free hamster ova in vitro, and higher than usual bacterial contamination of his semen was implicated as the probable cause. Findings suggested vigorous progressive sperm motility and acrosome integrity to be important features of good sperm samples. In one experiment a 4-cell stage embryo was transferred with the result that the recipient gave birth to a normal bull calf on June 9, 1981. The first calf resulting from in vitro fertilization has been found to be completely normal.

Benjamin G. Brackett

Daniel Bousquet

Melinda L. Boice

William J. Donawick

James F. Evans

Michael A. Dressel

Biology of Reproduction

生殖生物学 (BOR) 是生殖研究学会的官方期刊，发表关于生殖生物学领域广泛主题的原创研究，以及对当前重要或争议主题的评论。 BOR 一直是发表生殖生物学领域原创研究的引用率最高的期刊之一。

Biology of Reproduction is the official journal of the Society for the Study of Reproduction, publishing original research on a wide range of topics in the field of reproductive biology, as well as commentary on current important or controversial issues. It has consistently been one of the most highly cited journals for original research in the field of reproductive biology.

《Biology of Reproduction》是生殖研究学会的官方期刊，发表关于生殖生物学领域的原创研究，涵盖广泛的主题，并对当前重要或有争议的议题进行评论。该期刊一直是生殖生物学领域中引用率最高的原创研究发表平台之一。

Normal Development Following In Vitro Fertilization in the Cow

Findings suggested vigorous progressive sperm motility and acrosome integrity to be important features of good sperm samples and the first calf resulting from in vitro fertilization has been found to be completely normal.

Category	Quartile
生物学	2
生物学, 生殖生物学	3

Normal Development Following In Vitro Fertilization in the Cow

ivySCI AI Smartly Parses PDF, Answers Researchers' Questions, and Helps You Understand Papers in Seconds

Journal Info

Category	Quartile
REPRODUCTIVE BIOLOGY	2