Drug delivery technologies, which are a crucial area of research in the field of cell biology, aim to actively or passively deliver drugs to target cells to enhance therapeutic efficacy and minimize off-target effects. In recent years, with advances in drug development, particularly, the increasing demand for macromolecular drugs (e.g., proteins and nucleic acids), novel drug delivery technologies and intracellular cargo delivery systems have emerged as promising tools for cell and gene therapy. These systems include various viral- and chemical-mediated methods as well as physical delivery strategies. Physical methods, such as electroporation and microinjection, have shown promise in early studies but have not been widely adopted due to concerns regarding efficiency and cellular viability. Recently, microfluidic technologies have provided new opportunities for cargo delivery by allowing for precise control of fluid dynamic parameters to achieve efficient and safe penetration of cell membranes, as well as for foreign material transport. Microfluidics-based mechanical delivery methods utilize biophysical phenomena, such as cell constriction and fluid shear, and are associated with high throughput and high transfection efficiency. In this review, we summarize the latest advancements in microfluidic mechanical delivery technologies, and we discuss constriction- and fluid shear-induced delivery strategies. Furthermore, we explore the potential application of artificial intelligence in optimizing cargo delivery technologies, aiming to provide theoretical support and practical guidance for the future development of novel cellular drug delivery technologies.

Zhiyu Mao

Bori Shi

Jinbo Wu

Xinghua Gao

Biomicrofluidics

BIOMICROFLUIDICS (BMF) 是 AIP PUBLISHING 出版的仅限在线期刊，旨在快速传播与微流体和纳米流体现象相关的基本物理化学机制的研究。 BMF 还发表用于诊断、医学、生物、制药、环境和化学应用的独特微流体和纳米流体技术的研究。BMF 提供快速出版、多媒体功能以及在学术、国家和工业实验室之间的全球流通。 BMF 主要关注高质量的原创研究文章，还组织了专门的章节，帮助解释和定义生物微流体跨学科领域特有的特定挑战。微流体和纳米流体驱动（电动、声流体、光流体、毛细管）液体活检（MICRORNA 分析、循环肿瘤细胞分离、外泌体分离、循环肿瘤DNA定量）细胞分选、操作和转染（DI/电泳、磁珠、光阱、电穿孔）分子分离和浓缩（等速电泳、浓差极化、DI/电泳、磁珠, 纳米颗粒）细胞培养和分析（单细胞测定、刺激反应、干细胞转染）基因组和蛋白质组分析（快速基因测序、DNA/蛋白质/碳水化合物阵列）生物传感器（免疫测定、核酸荧光测定、比色测定、酶放大，等离子体和拉曼纳米报告分子，分子 B EACON、FRET、适配体、纳米孔、光纤）生物物理传输和表征（DNA、单蛋白、离子通道和膜动力学、细胞运动和通信机制、电生理学、膜片钳）。 ETC...

Biomicrofluidics is an online journal published by AIP PUBLISHING, aimed at the rapid dissemination of research related to the fundamental physicochemical mechanisms of microfluidic and nanofluidic phenomena. The journal also publishes unique microfluidic and nanofluidic technologies for applications in diagnostics, medicine, biology, pharmaceuticals, environmental science, and chemistry. It offers rapid publication, multimedia features, and global circulation among academic, national, and industrial laboratories. Biomicrofluidics primarily focuses on high-quality original research articles and organizes dedicated sections to help explain and define the specific challenges unique to the interdisciplinary field of biomicrofluidics. Research topics include microfluidic and nanofluidic-driven liquid biopsies (such as MICRORNA analysis, circulating tumor cell isolation, exosome separation, and circulating tumor DNA quantification), cell sorting, manipulation, and transfection (such as DI/electrophoresis, magnetic beads, optical traps, and electroporation), molecular separation and concentration (such as isoelectric focusing, concentration polarization, DI/electrophoresis, magnetic beads, and nanoparticles), cell culture and analysis (such as single-cell assays, stimulation responses, and stem cell transfection), genomics and proteomics (such as rapid gene sequencing, DNA/protein/carbohydrate arrays), biosensors (such as immunoassays, nucleic acid fluorescence assays, colorimetric assays, enzyme amplification, plasmonic and Raman nano-reporters, molecular B EACON, FRET, aptamers, nanopores, and optical fibers), and biophysical transport and characterization (such as DNA, single proteins, ion channels and membrane dynamics, cell motility and communication mechanisms, electrophysiology, and patch clamping).

《Biomicrofluidics》是AIP PUBLISHING出版的在线期刊，旨在快速传播与微流体和纳米流体现象相关的基本物理化学机制的研究。该期刊还发表用于诊断、医学、生物、制药、环境和化学应用的独特微流体和纳米流体技术的研究。期刊提供快速出版、多媒体功能，并促进学术界、国家和工业实验室之间的全球交流。《Biomicrofluidics》主要关注高质量的原创研究文章，并组织专门章节，以帮助解释和定义生物微流体领域特有的挑战。研究主题包括微流体和纳米流体驱动的液体活检（如MICRORNA分析、循环肿瘤细胞分离、外泌体分离、循环肿瘤DNA定量）、细胞分选、操作和转染（如DI/电泳、磁珠、光阱、电穿孔）、分子分离和浓缩（如等速电泳、浓差极化、DI/电泳、磁珠、纳米颗粒）、细胞培养和分析（如单细胞测定、刺激反应、干细胞转染）、基因组和蛋白质组分析（如快速基因测序、DNA/蛋白质/碳水化合物阵列）、生物传感器（如免疫测定、核酸荧光测定、比色测定、酶放大、等离子体和拉曼纳米报告分子、分子B EACON、FRET、适配体、纳米孔、光纤）以及生物物理传输和表征（如DNA、单蛋白、离子通道和膜动力学、细胞运动和通信机制、电生理学、膜片钳等）。

Category	Quartile
工程技术	4
工程技术, 生化研究方法	4
工程技术, 生物物理	4
工程技术, 纳米科技	4
工程技术, 物理流体与等离子体	4

Mechanically mediated cargo delivery to cells using microfluidic devices

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Journal Info

Category	Quartile
PHYSICS, FLUIDS & PLASMAS	2