The genetic improvement of trees for freezing tolerance is one of the most important goals to extend the plantations to colder areas. RNA-Seq technology has become a key tool in transcriptome studies. It can quantify overall expression levels for each gene simultaneously with high efficiency and speed through in silico gene expression, where differentially expressed genes can be identified by measuring the reads mapped for each transcript. In this study, the results of ESTs libraries from two Eucalyptus globulus genotypes showing contrasting differences in frost tolerance after cold acclimation using mRNA-Seq and in silico gene expression are discussed. A total of 14,265 non-redundant transcripts were predicted, where 163 corresponded to upregulated and 537 to downregulated genes. Pathway analyses of upregulated transcripts indicated that differences in frost tolerance might be regulated by the tree response to chemical and osmotic stimulus and organic substances, principally by overexpressing proteins that respond to hormone stress. These results suggest that genes coding for dehydrins, outer envelope, and voltage-dependent anion channel proteins are likely to participate in the regulation of the cold acclimation process and may have an important role in frost tolerance. The transcription factor analysis allowed identifying that those most differentially expressed in a resistant genotype were participating in the regulation of transcription, hormone regulation, photosynthesis, and response to stress. Additionally, the screening of polymorphic EST-SSR in silico and the validation of these markers in a reference population lead to identify a polymorphic EST-SSR with potential use for plant breeding and genotype discrimination.

Marta Fernández

Valentina Troncoso

Sofia Valenzuela

Plant Molecular Biology Reporter

PLANT MOLECULAR BIOLOGY REPORTER 杂志的范围已经扩大，以跟上分子生物学和基因组学广泛领域的新发展。该杂志现在征集的论文涵盖分子生物学、基因组学、蛋白质组学、代谢组学和其他“组学”以及生物信息学领域的无数突破性技术和发现。

The scope of the Plant Molecular Biology Reporter has expanded to keep pace with new developments in the broad fields of molecular biology and genomics. The journal now invites submissions covering numerous groundbreaking technologies and discoveries in molecular biology, genomics, proteomics, metabolomics, and other 'omics' fields, as well as research in bioinformatics.

《Plant Molecular Biology Reporter》杂志的范围已扩展，以跟上分子生物学和基因组学广泛领域的新发展。该杂志现在征集的论文涵盖分子生物学、基因组学、蛋白质组学、代谢组学及其他“组学”领域的众多突破性技术和发现，以及生物信息学的相关研究。

Transcriptome Profile in Response to Frost Tolerance in Eucalyptus globulus

Results suggest that genes coding for dehydrins, outer envelope, and voltage-dependent anion channel proteins are likely to participate in the regulation of the cold acclimation process and may have an important role in frost tolerance.

Category	Quartile
生物学	4
生物学, 生化研究方法	4
生物学, 植物科学	4

Transcriptome Profile in Response to Frost Tolerance in Eucalyptus globulus

ivySCI AI Smartly Parses PDF, Answers Researchers' Questions, and Helps You Understand Papers in Seconds

Journal Info