Seven bacteria strains that have a comparable or higher cellulose degrading capability than Cellulomonas fimi were screened out and phylogenetically analyzed. Genes encoding 1,4-endoglucosidase (celJV) and glucoside hydrolase family 8 (celG) were cloned from Klebsiella sp. strain IV-3, and then inserted separately or simultaneously into pET-22b(+), pETDuet-1 (pDin) or pDout that combined pETDuet-1 with the pelB leader. pETDuet-1 expressed these cellulose genes as soluble protein, while other vectors mainly as inclusion bodies. The recombinant enzyme(s) from pDin-JV-G and pDin-JV had the highest degrading capability to CMC-Na, with the degrading zone diameter corresponding to 2.6- and 1.9-fold of Klebsiella sp. strain IV-3, respectively. They also showed the highest degrading capability to filter paper, which corresponded well with the highest specific activity. Even though pDin-JV-G had lower expression efficiency, it still had higher degrading capability than pDin-JV, indicating that enzymatic synergism is helpful for cellulose degradation. Key words: Cellulose, cellulase, 1,4-endoglucosidase, glucoside hydrolase family 8, Klebsiella.

Li Qian

Hong Xiao

Chen Xiwen

Chen Defu

African Journal of Microbiology Research

Expression and characterization of recombinant cellulases from an isolated Klebsiella

Seven bacteria strains that have a comparable or higher cellulose degrading capability than Cellulomonas fimi were screened out and phylogenetically analyzed, indicating that enzymatic synergism is helpful for cellulose degradation.

Expression and characterization of recombinant cellulases from an isolated Klebsiella

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