Fluorescently labeled and conjugated (strept)avidins are widely used for imaging biotinylated molecules in immunological assays and histochemistry. We showed that besides biotin, these proteins bind glycans, including fragments of mammalian glycoproteins and glycolipids, in particular, ABO blood group antigens, oligolactosamines, and 6-O-sulfated oligosaccharides. This interaction is inhibited in a dose-dependent manner by micromolar concentrations of polymeric, but not monomeric, glycan conjugates (i.e., requires polyvalence). Taking into account the cluster organization of cell glycans (glycoproteins and glycolipids), the ability of (strept)avidins to bind glycans might be a source of errors in the analysis of carbohydrate-containing samples, which can be prevented by avoiding a large excess of (strept)avidin in analytical systems.

Nadezhda V Shilova

Svetlana M Polyakova

Alexey Yu Nokel

Alexander D Lipatnikov

Elena A Gordeeva

Marina V Lavrenteva

Nicolai V Bovin

Biochemistry (Moscow)

BIOCHEMISTRY (MOSCOW) 是一本收录生物化学所有领域以及分子生物学、生物有机化学、微生物学、免疫学、生理学和生物医学科学的生化方面的研究论文的期刊。报道还延伸到生物化学的新实验方法、生化重要性的理论贡献、当代生化主题的评论和小型评论（生物化学新闻）。

Biochemistry (Moscow) is a journal that covers all areas of biochemistry, including research papers in molecular biology, bioorganic chemistry, microbiology, immunology, physiology, and biomedical sciences. The journal also reports on new experimental methods in biochemistry, theoretical contributions of significant importance, and reviews and brief commentaries on contemporary biochemical topics (Biochemistry News).

《Biochemistry (Moscow)》是一本涵盖生物化学各个领域的期刊，涉及分子生物学、生物有机化学、微生物学、免疫学、生理学和生物医学科学的研究论文。期刊内容还包括生物化学的新实验方法、理论贡献以及当代生化主题的评论和简短综述（生物化学新闻）。

Category	Quartile
生物学	4
生物学, 生化与分子生物学	4

(Strept)avidin Binds Glycoconjugates

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