Outcomes for patients with metastatic and recurrent osteosarcoma remain poor and a better understanding of the biology of this malignancy is critical to the development of prognostic biomarkers and novel therapies. The purpose of this study was to establish a biobank of osteosarcoma which has the potential of monitoring tumors dynamically with exosomes, to facilitate clinical and basic scientific research. The osteosarcoma biological specimen and clinical data of osteosarcoma were collected in Ruijin Hospital in two stages. In the first stage (2015-2017), the collection of tissue specimens and blood samples were performed at diagnostic biopsy, definitive surgery, recurrence and lung metastasis, according to the Children's Oncology Group protocol. In the second stage (2017-2019), the tissue specimens were collected the same as before, but the blood samples were collected at the beginning of each MAP-I (methotrexate, cisplatin, doxorubicin, ifosfamide) chemotherapy cycle, and every 6 months after the last chemotherapy up to 3 years, according to our modified protocol, to dynamically monitor the status of possible alteration of gene expression profiling in the osteosarcoma. A total of 268 patients with osteosarcoma were enrolled in this study, 161 were men and 107 were women, with the mean age of 24.51 ± 15.58 years. Local recurrence occurred in 29 patients and lung metastasis in 51. The numbers of tissue and blood specimens reached 360 and 1023, respectively. 11 specimens were from recurrent osteosarcoma and 25 were from lung metastasis. The corresponding clinical and demographic data were collected in our electronic database. The osteosarcoma biobank built with our modified protocol mentioned above has the potential of monitoring tumors dynamically with exosomes and could provide specimens to the researches improving the biological understanding and outcome of this disease.

Shizhao Zang

Jun Wang

Junxiang Wen

Qiyuan Bao

Yuhui Shen

Weibin Zhang

Cell and tissue banking

细胞和组织库提供了一个向参与细胞和组织库和移植的科学家和临床医生传播信息的论坛。 CELL AND TISSUE BANKING 是一份国际同行评审期刊，发表以下领域的原创论文：关于组织银行一般方面的基础研究，例如储存细胞/组织的质量保证和控制、保存和灭菌方法对细胞/组织、生物技术等；储存细胞/组织的临床应用；细胞/组织采购、加工、储存和分配的实践标准；伦理道德问题;法医问题。

Cell and Tissue Banking is an international peer-reviewed journal that provides a forum for scientists and clinicians involved in cell and tissue banking and transplantation to disseminate information. The journal publishes original research papers in the following areas: fundamental research on general aspects of tissue banking, such as quality assurance and control for the storage of cells/tissues, the effects of preservation and sterilization methods on cells/tissues, biotechnology, etc.; clinical applications of stored cells/tissues; practical standards for the procurement, processing, storage, and distribution of cells/tissues; ethical issues; and forensic matters.

《Cell and Tissue Banking》是一个国际同行评审期刊，旨在为参与细胞和组织库及移植的科学家和临床医生提供一个信息交流的平台。期刊发表原创研究论文，涵盖以下领域：关于组织银行的基础研究，包括细胞和组织的质量保证与控制、保存和灭菌方法对细胞和组织的影响、生物技术等；细胞和组织的临床应用；细胞和组织的采购、加工、储存和分配的实践标准；伦理道德问题以及法医问题。

Category	Quartile
医学	4
医学, 细胞生物学	4
医学, 工程生物医学	4

Establishment of a dynamic osteosarcoma biobank: Ruijin experience.

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Journal Info

Category	Quartile
ENGINEERING, BIOMEDICAL	4