Abstract Febrifugine, a potent quinazolinone compound derived from the Chinese herb Chang Shan (Dichroa febrifuga), exhibits diverse biological activities and has demonstrated anti-tumor effects by functioning as focal adhesion kinase (FAK) inhibitors. In this study, our objective was to establish a quantitative UPLC-MS/MS method and investigate the pharmacokinetic characteristics of febrifugine in rats following intravenous and oral administration routes. The rat tail vein was used for the collection of blood samples at designated time intervals following intravenous (2.0 mg kg −1 ) and oral (6.0 mg kg −1 ) administrations. Plasma samples were pretreated with acetonitrile as a protein precipitant and methylcytisine as an internal standard. Febrifugine concentration in rat plasma was determined using the UPLC-MS/MS method, and pharmacokinetic parameters were calculated using drug and statistics (DAS) software version for statistical analysis. The linear range of febrifugine in rat plasma was 1.5–1,500 ng mL −1 , meeting the precision, recovery, and stability requirements for determination purposes. Febrifugine had a half-life (t 1/2 ) of 3.2 ± 1.6 h after administered via intravenous route, while t 1/2 was 2.6 ± 0.5 h after oral administration. The developed UPLC-MS/MS method is facile to operate while adhering to rigorous methodological verification standards, rendering it suitable for investigating the pharmacokinetics of febrifugine; and bioavailability was determined as 45.8%.

Feifei Lin

Runrun Wang

Jingye Du

Congcong Wen

Xianqin Wang

Yongxi Jin

Lingjiu Shao

Acta Chromatographica

ACTA CHROMATOGRAPHICA 开放存取ACTA CHROMATOGRAPHICA 发表关于色谱各个领域的同行评审科学文章，包括色谱理论；新固定相的合成和表征进展；有机、无机和复杂化合物的色谱分析；手性化合物的对映体分离和色谱分析；色谱在生物学、药学、医学和食品分析中的应用；色谱的环境应用；色谱样品制备的分析和物理化学方面；连字符和组合技术；化学计量学及其在分离科学中的应用。

Acta Chromatographica is an open-access journal that publishes peer-reviewed scientific articles in the field of chromatography. The content covers chromatography theory, the synthesis and characterization of new stationary phases, chromatographic analysis of organic, inorganic, and complex compounds, enantiomer separation and analysis of chiral compounds, applications of chromatography in biology, pharmacy, medicine, and food analysis, environmental applications of chromatography, analysis and physicochemical aspects of chromatographic sample preparation, hyphenated and combined techniques, and the application of chemometrics in separation science.

《Acta Chromatographica》是一份开放获取的期刊，发表关于色谱领域的同行评审科学文章。内容涵盖色谱理论、新型固定相的合成与表征、有机、无机及复杂化合物的色谱分析、手性化合物的对映体分离与分析、色谱在生物学、药学、医学及食品分析中的应用、色谱的环境应用、色谱样品制备的分析与物理化学方面、连锁与组合技术、以及化学计量学在分离科学中的应用等。

Category	Quartile
化学	4
化学, 分析化学	4

Pharmacokinetics and bioavailability of febrifugine in rat plasma determined by UPLC-MS/MS

ivySCI AI Smartly Parses PDF, Answers Researchers' Questions, and Helps You Understand Papers in Seconds

Journal Info

Category	Quartile
CHEMISTRY, ANALYTICAL	3