ABSTRACT Acetylcholine (ACh), a vital neurotransmitter for both the peripheral (PNS) and central nervous systems (CNS), signals through nicotinic ACh receptors (nAChRs) and muscarinic ACh receptors (mAChR). Here, we explore the expression patterns of three nAChR subunits, chrna3 , chrnb4 , and chrna5 , which are located in an evolutionary conserved cluster. This close genomic positioning, in a range of vertebrates, may indicate co‐functionality and/or co‐expression. Through novel transgenic zebrafish lines, we observe widespread expression within both the PNS and CNS. In the PNS, we observed expression of chrna3 tdTomato , chrnb4 eGFP , and chrna5 tdTomato in the intestinal enteric nervous system; chrna5 tdTomato and chrnb4 eGFP in sensory ganglia of the lateral line; and chrnb4 eGFP in the ear. In the CNS, the expression of chrnb4 eGFP and chrna5 tdTomato was found in the retina, all three expressed in diverse regions of the brain, where a portion of chrna3 tdTomato and chrnb4 eGFP cells were found to be inhibitory efferent neurons projecting to the lateral line. Within the spinal cord, we identify distinct populations of chrna3 tdTomato ‐, chrnb4 eGFP ‐, and chrna5 tdTomato ‐expressing neurons within the locomotor network, including dmrt3a ‐expressing interneurons and mnx1 ‐expressing motor neurons. Notably, three to four primary motor neurons per hemisegment were labeled by both chrna3 tdTomato and chrnb4 eGFP . Interestingly, we identified an sl‐type secondary motor neuron per hemisegement that strongly expressed chrna5 tdTomato and co‐expressed chrnb4 eGFP . These transgenic lines provide insights into the potential roles of nAChRs within the locomotor network and open avenues for exploring their role in nicotine exposure and addiction in a range of tissues throughout the nervous system.

Yuanqi Hua

Judith Habicher

Matthias Carl

Remy Manuel

Henrik Boije

Developmental Neurobiology

发育神经生物学（前身为《神经生物学杂志》）发表关于神经系统发育、再生、修复和可塑性以及行为个体发育的原创研究文章。征集这些领域的高质量贡献，强调实验而不是纯粹的描述性工作。该杂志还将考虑报告用于研究神经系统发育的新方法和技术的手稿，以及关于当前重要主题的偶尔特刊。我们欢迎读者就可能的主题提出建议。

Developmental Neurobiology is a scholarly journal focused on original research articles concerning the development, regeneration, repair, and plasticity of the nervous system, as well as the developmental behavior of individuals (formerly known as the Journal of Neurobiology). We welcome high-quality contributions in these areas, emphasizing experimental work over purely descriptive studies. The journal also considers manuscripts reporting new methods and techniques for researching nervous system development, as well as occasional special issues on current important topics. We encourage readers to suggest potential themes.

《Developmental Neurobiology》是一本专注于神经系统发育、再生、修复和可塑性以及行为个体发育的学术期刊（前身为《神经生物学杂志》）。我们欢迎在这些领域内的高质量原创研究文章，特别强调实验研究而非纯描述性工作。此外，本期刊也会考虑关于神经系统发育的新方法和技术的报告，以及偶尔发布关于当前重要主题的特刊。我们鼓励读者提出可能的主题建议。

Category	Quartile
医学	4
医学, 发育生物学	4
医学, 神经科学	4

Novel Transgenic Zebrafish Lines to Study the CHRNA3‐B4‐A5 Gene Cluster

ivySCI AI Smartly Parses PDF, Answers Researchers' Questions, and Helps You Understand Papers in Seconds

Journal Info