Peroxisome proliferator-activated receptor-γ (PPARγ) is the master regulator of adipogenesis, but knowledge about how PPARγ is regulated at the protein level is very limited. We aimed to identify PPARγ-interacting proteins which modulate PPARγ’s protein levels and transactivating activities in human adipocytes. We expressed Flag-tagged PPARγ in human preadipocytes as bait to capture PPARγ-associated proteins, followed by mass spectroscopy and proteomics analysis, which identified serine/threonine kinase 38 (STK38) as a major PPARγ-associated protein. Protein pulldown studies confirmed this protein–protein interaction in transfected cells, and reporter assays demonstrated that STK38 enhanced PPARγ’s transactivating activities without requiring STK38’s kinase activity. In cell-based assays, STK38 increased PPARγ protein stability, extending PPARγ’s half-life from ~1.08 to 1.95 h. Notably, in human preadipocytes, the overexpression of STK38 enhanced adipogenesis, whereas knockdown impaired the process in a PPARγ-dependent manner. Thus, we discovered that STK38 is a novel PPARγ-cofactor promoting adipogenesis, likely through stabilization of PPARγ

Kun Qian

Daozhan Yu

Weiming Wang

Mengqi Jiang

Rongze Yang

Robert Brown

Da-Wei Gong

Adipocyte

脂肪细胞认识到脂肪组织是体内最大的内分泌器官，并探索功能失调的脂肪组织与越来越多的慢性疾病（包括糖尿病、高血压、心血管疾病和癌症）之间的联系。从历史上看，脂肪组织的主要功能仅限于能量储存和体温调节。然而，过去 3 年的大量研究已经认识到脂肪组织的动态作用及其对多种生理过程的贡献，包括生殖、血管生成、细胞凋亡、炎症、血压、凝血、纤维蛋白溶解、免疫和一般代谢稳态.脂肪组织研究领域取得了巨大的发展，《脂肪细胞》是第一个国际同行评审期刊，为研究提供了一个多学科论坛，专门关注脂肪组织生理学和病理生理学的各个方面。脂肪细胞接受基础、转化和临床研究方面的高调提交。

The journal "Adipocyte" recognizes adipose tissue as the largest endocrine organ in the body and explores the connections between dysfunctional adipose tissue and an increasing number of chronic diseases, including diabetes, hypertension, cardiovascular diseases, and cancer. Historically, the primary functions of adipose tissue were limited to energy storage and thermoregulation. However, extensive research over the past few years has revealed the dynamic roles of adipose tissue and its contributions to various physiological processes, including reproduction, angiogenesis, apoptosis, inflammation, blood pressure regulation, coagulation, fibrinolysis, immune response, and overall metabolic homeostasis. The field of adipose tissue research has made significant advancements, and "Adipocyte" is the first international peer-reviewed journal that provides a multidisciplinary forum dedicated to all aspects of adipose tissue physiology and pathophysiology. The journal welcomes high-quality submissions in basic, translational, and clinical research.

《Adipocyte》期刊认识到脂肪组织是体内最大的内分泌器官，并探索功能失调的脂肪组织与越来越多的慢性疾病（如糖尿病、高血压、心血管疾病和癌症）之间的关系。历史上，脂肪组织的主要功能仅限于能量储存和体温调节。然而，近年来的研究表明，脂肪组织在多种生理过程中发挥着动态作用，包括生殖、血管生成、细胞凋亡、炎症、血压调节、凝血、纤维蛋白溶解、免疫反应和整体代谢稳态。脂肪组织研究领域取得了显著进展，《Adipocyte》是首个国际同行评审期刊，为研究者提供了一个多学科的论坛，专注于脂肪组织生理学和病理生理学的各个方面。该期刊欢迎基础、转化和临床研究的高质量投稿。

STK38 is a PPARγ-interacting protein promoting adipogenesis

It is discovered that STK38 is a novel PPARγ-cofactor promoting adipogenesis, likely through stabilization of PPARαγ, through interaction with serine/threonine kinase 38.

Category	Quartile
生物学	4
生物学, 内分泌学与代谢	4

STK38 is a PPARγ-interacting protein promoting adipogenesis

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Journal Info

Category	Quartile
ENDOCRINOLOGY & METABOLISM	2