In the present study, the effects of trans-MUFA, elaidic acid (EA; 18 : 1-9t) and vaccenic acid (VA; 18 : 1-11t) on rat neutrophil functions were compared with those of cis-monounsaturated oleic acid (OA) (18 : 1-9c) and saturated stearic acid (SA; 18 : 0) (10–150 μm). Trans-fatty acids enhanced neutrophil phagocytic capacity, superoxide (O2∙-) and hydrogen peroxide production, and candidacidal activity. The same effects were observed for OA. Cells treated with trans-MUFA showed reduced production of NO∙, whereas those treated with OA showed an increase in production. Treatment with SA did not provoke significant effect on the parameters investigated. The increase in O2∙- production induced by MUFA was not observed when diphenyleneiodonium, an NADPH oxidase inhibitor, was added to the medium. This finding suggests that MUFA stimulate neutrophil NADPH oxidase activity. The addition of 3-[1-[3-(dimethylamino)propyl]-1H-indol-3-yl]-4-(1H-indol-3-yl)-1H-pyrrole-2,5-dione, a protein kinase C (PKC) inhibitor, and wortmannin, a phosphatidylinositol-3 kinase (PI3K) inhibitor, did not affect O2∙- production induced by MUFA. Therefore, the mechanisms by which MUFA stimulate NADPH oxidase are not dependent on PKC and do not seem to involve PI3K. Experiments using Zn2+, an inhibitor of NADPH oxidase H+ channel, indicated that MUFA activate the NADPH oxidase complex in rat neutrophil due to opening of H+ channel.

Renato Padovese

Rui Curi

British Journal Of Nutrition

BRITISH JOURNAL OF NUTRITION 是一本领先的国际同行评审期刊，涵盖人类和临床营养、动物营养和营养学基础科学的研究。该杂志承认营养科学的多学科性质，包括来自营养研究的所有专业的材料，包括分子和细胞生物学以及营养基因组学。

The British Journal of Nutrition is a leading international peer-reviewed journal that focuses on research in human and clinical nutrition, animal nutrition, and the fundamental sciences of nutrition. The journal recognizes the multidisciplinary nature of nutritional science, encompassing materials from all specialties within nutritional research, including molecular and cellular biology as well as nutritional genomics.

《British Journal of Nutrition》是一本国际领先的同行评审期刊，专注于人类和临床营养、动物营养以及营养学基础科学的研究。该期刊承认营养科学的多学科特性，涵盖来自营养研究各个领域的研究成果，包括分子和细胞生物学以及营养基因组学。

British Journal of Nutrition

Modulation of rat neutrophil function in vitro by cis- and trans-MUFA

Experiments indicated that MUFA activate the NADPH oxidase complex in rat neutrophil due to opening of H+ channel, and the mechanisms by which MUFA stimulate NADPH oxidation are not dependent on PKC and do not seem to involve PI3K.

Category	Quartile
医学	3
医学, 营养学	3

Modulation of rat neutrophil function in vitro by cis- and trans-MUFA

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