The Lactate Shuttle concept has revolutionized our understanding and study of metabolism in physiology, biochemistry, metabolism, nutrition, and medicine. Seminal findings of the Mitochondrial Lactate Oxidation Complex (mLOC) elucidated the architectural structure of its components. Here, we report that the mitochondrial pyruvate carrier (mPC) is an additional member of the mLOC in mouse muscle and C2C12 myoblasts and myotubes. Immunoblots, mass spectrometry, and co-immunoprecipitation experiments of mitochondrial preparations revealed abundant amounts of mitochondrial lactate dehydrogenase (mLDH), monocarboxylate transporter (mMCT), basigin (CD147), cytochrome oxidase (COx), and pyruvate carriers 1 and 2 (mPC1 and 2). Additionally, using confocal laser scanning microscopy (CLSM) and in situ proximity ligation, we also demonstrated planar and 3D colocalization of pyruvate and lactate transporters with COx in fixed skeletal muscle sections, myotubes, and C2C12 myoblasts. This work serves as a landmark for configuring the final pathway of carbohydrate oxidation.

Robert G. Leija

Jose A. Arevalo

Dianna Xing

José Pablo Vázquez-Medina

George A. Brooks

AJP Endocrinology and Metabolism

AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM 发表关于内分泌和代谢系统生理学的原创性机械研究。将考虑对整个动物或人类进行的生理、细胞和分子研究。具体主题包括但不限于激素和生长因子作用机制；新陈代谢、炎症、微生物组和能量平衡的激素和营养调节；综合器官相声；内分泌细胞的旁分泌和自分泌控制；激素受体的功能和激活；通道、转运蛋白和膜功能的内分泌或代谢控制；激素分泌和代谢的时间分析；和代谢的数学/动力学模型。也欢迎对激素作用进行新的分子、免疫学或生物物理学研究。

The American Journal of Physiology - Endocrinology and Metabolism publishes original research on the physiology of the endocrine and metabolic systems. We welcome physiological, cellular, and molecular studies conducted on whole animals or humans. Specific topics include, but are not limited to: mechanisms of action of hormones and growth factors; hormonal and nutritional regulation of metabolism, inflammation, the microbiome, and energy balance; interactions among integrated organs; paracrine and autocrine control of endocrine cells; functions and activation of hormone receptors; endocrine or metabolic control of channels, transport proteins, and membrane functions; temporal analysis of hormone secretion and metabolism; and mathematical/dynamic modeling of metabolism. We also welcome new molecular, immunological, or biophysical studies on hormone actions.

《American Journal of Physiology - Endocrinology and Metabolism》发表关于内分泌和代谢系统生理学的原创性研究。我们欢迎对整个动物或人类进行的生理、细胞和分子研究，具体主题包括但不限于：激素和生长因子的作用机制；新陈代谢、炎症、微生物组和能量平衡的激素与营养调节；综合器官的相互作用；内分泌细胞的旁分泌和自分泌控制；激素受体的功能与激活；通道、转运蛋白和膜功能的内分泌或代谢控制；激素分泌和代谢的时间分析；以及代谢的数学和动力学模型。此外，我们也欢迎对激素作用的新分子、免疫学或生物物理学研究。

Category	Quartile
医学	2
医学, 内分泌学与代谢	2
医学, 生理学	2

The Mitochondrial Lactate Oxidation Complex: endpoint for carbohydrate carbon disposal

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