Cells and tissues are the foundation of translational medicine. At present, one of the main technological obstacles is their preservation for long-term usage while maintaining adequate viability and function. Optimized storage techniques must be developed to make them safer to use in the clinic. Cryopreservation is the most common long-term preservation method to maintain the vitality and function of cells and tissues. But, the formation of ice crystals in cells and tissues is considered to be the main mechanism that could harm cells and tissues during freezing and thawing. To reduce the formation of ice crystals, cryoprotective agents (CPAs) must be added to the cells and tissues to achieve the cryoprotective effect. However, conventional cryopreservation of cells and tissues often needs to use toxic organic solvents as CPAs. As a result, cryopreserved cells and tissues may need to go through a time-consuming washing process to remove CPAs for further applications in translational medicine, and multiple valuable cells are potentially lost or killed. Currently, trehalose has been researched as a nontoxic CPA due to its cryoprotective ability and stability during cryopreservation. Nevertheless, trehalose is a nonpermeable CPA, and the lack of an effective intracellular trehalose delivery method has become the main obstacle to its use in cryopreservation. This article illustrated the properties, mechanisms, delivery methods, and applications of trehalose, summarized the benefits and limits of trehalose, and summed up the findings and research direction of trehalose in biomedical cryopreservation.

Yuying Hu

Xiangjian Liu

Fenglin Liu

Jingxian Xie

Qubo Zhu

Songwen Tan

ACS Biomaterials Science & Engineering

Modeling and informatics tools for biomaterials – scaling methods to guide biomaterial design, predictive algorithms for structure-function, biomaterial mechanics; integrating bioinformatics with biomaterials discovery, metabolomics in the context of biomaterials

New biomaterials, bioinspired and biomimetic approaches to biomaterials – synthesis and chemical modifications, exploiting structural hierarchy and architectural control, combinatorial strategies for biomaterials discovery, new processing modes to purify and utilize natural polymers, biophysical regulation of cell functions, controlled and bioresponsive delivery of regulatory molecules, genetic biomaterials design, synthetic biology, new composite systems

Biomaterial interfaces, biology and health - material-biology interactions, chemical/morphological/structural communication, signaling and biological responses, health risks, toxicology, safety, calcification, corrosion and degradation of biomaterials and devices, prosthetics

Manufacturing, technology and tissues in the context of biomaterials – regenerative medicine, tissue engineering for basic and applied studies, organ-on-a-chip, bioreactor/perfusion systems, microdevices, 3D printing, inks, BioMEMS, optics and electronics interfaces with biomaterials, systems integration

The journal "ACS Biomaterials Science and Engineering" focuses on modeling and informatics tools for biomaterials, covering scaling methods to guide biomaterial design, predictive algorithms for structure-function relationships, and biomaterial mechanics. It also explores new biomaterials and bioinspired approaches, including synthesis and chemical modifications, exploiting structural hierarchy and architectural control, combinatorial strategies for biomaterials discovery, and new processing modes to purify and utilize natural polymers. Additionally, the journal addresses biophysical regulation of cell functions, controlled and bioresponsive delivery of regulatory molecules, genetic biomaterials design, synthetic biology, and new composite systems. The interactions between biomaterial interfaces and biology and health are also examined, including material-biology interactions, chemical/morphological/structural communication, signaling and biological responses, health risks, toxicology, safety, calcification, corrosion, and degradation of biomaterials and devices, as well as issues related to prosthetics. Finally, the journal discusses manufacturing, technology, and tissues in the context of biomaterials, including regenerative medicine, tissue engineering for basic and applied studies, organ-on-a-chip, bioreactor/perfusion systems, microdevices, 3D printing, inks, BioMEMS, optics and electronics interfaces with biomaterials, and systems integration.

《ACS Biomaterials Science and Engineering》期刊专注于生物材料的建模与信息工具，涵盖了指导生物材料设计的缩放方法、结构-功能的预测算法、生物材料力学等内容。同时，期刊还探讨了生物材料的新型合成及化学改性，利用结构层次和建筑控制的生物启发和仿生方法，组合策略用于生物材料的发现，以及新型处理模式以提纯和利用天然聚合物。此外，期刊关注细胞功能的生物物理调控、调节分子的受控和生物响应递送、遗传生物材料设计、合成生物学和新复合系统。期刊还研究生物材料界面与生物学和健康的关系，包括材料与生物的相互作用、化学/形态/结构的沟通、信号传递和生物反应、健康风险、毒理学、安全性、钙化、腐蚀和生物材料及设备的降解，以及假肢的相关问题。最后，期刊涉及生物材料在制造、技术和组织中的应用，包括再生医学、基础与应用研究的组织工程、器官芯片、生物反应器/灌注系统、微设备、3D打印、墨水、生物微机电系统（BioMEMS）、与生物材料的光学和电子接口，以及系统集成。

ACS Biomaterials Science and Engineering

Trehalose in Biomedical Cryopreservation–Properties, Mechanisms, Delivery Methods, Applications, Benefits, and Problems

The properties, mechanisms, delivery methods, and applications of trehalose are illustrated, the benefits and limits are summarized, and the findings and research direction oftrehalose in biomedical cryopreservation are summed up.

Category	Quartile
医学	2
医学, 材料科学生物材料	3

Trehalose in Biomedical Cryopreservation–Properties, Mechanisms, Delivery Methods, Applications, Benefits, and Problems

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Journal Info

Category	Quartile
MATERIALS SCIENCE, BIOMATERIALS	2